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pcDNA3-HA-H-Ras_wt

Plasmid

#39503

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS (HRAS Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP C3-YAP2

Plasmid

#19055

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pCP60 RS-GFP

Plasmid

#122173

Purpose

"Binary vector for Agrobacterium tumefacience-mediated transformation of plant cells resulting in constitutive expression of cytoplasmatically localized RS-GFP"

Depositor

Lukas Fischer

Article

Nocarova et al BMC Plant Biol. 2009 Apr 22;9:44. doi: 1

Insert

RS-GFP

Use

Binary vector for escherichia coli and agrobacter…

Expression

Plant

Promoter

CaMV 35S

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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hE-cadherin/Δβ-catenin-pcDNA3

Plasmid

#45772

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

hE-cadherin/Δβ-catenin (CDH1 Human)

Expression

Mammalian

Mutation

Ends at amino acid 844. Last 35 amino acids dele…

Promoter

CMV

Available Since

June 14, 2013

Availability

Academic Institutions and Nonprofits only

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pMXs-IP spGFP-ERGIC53

Plasmid

#38270

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

ERGIC-53 (LMAN1 Human)

Use

Retroviral

Tags

EGFP with Calreticulin signal peptide at N-termin…

Expression

Mammalian

Mutation

deleted signal peptide (1-30 amino acids)

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pMCL-HA-MAPKK1-R4F [delta(31-51)/S218E/S222D]

Plasmid

#40810

Depositor

Natalie Ahn

Article

Mansour et al Science. 1994 Aug 12;265(5174):966-70.

Insert

MAP2K1 (MAP2K1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta(31-51), S218E, S222D

Promoter

CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only
pGWB555

Plasmid

#74885

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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HA-POMP

Plasmid

#86765

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

human POMP (POMP Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pHBS838 TDP43 RRM-GFP

Plasmid

#98249

Purpose

TDP43 RRM-GFP reporter construct

Depositor

Rajat Rohatgi

Article

Schmidt et al Cell Rep. 2016 Aug 2;16(5):1228-36. doi:

Insert

TARDBP (TARDBP Human)

Tags

Internal GFP to replace RRM domains

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tet2 CD

Plasmid

#72219

Purpose

Express flag-tagged mouse Tet2 catalytic domain.

Depositor

Yi Zhang

Article

Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

Insert

Ten-Eleven Translocation 2 (Tet2 Mouse)

Tags

flag

Expression

Mammalian

Mutation

The plasmid expresses TET2 catalytic domain.

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only
pBABE YAP1

Plasmid

#15682

Depositor

Joan Brugge

Article

Overholtzer et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 31, 2007

Availability

Academic Institutions and Nonprofits only

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pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI

Plasmid

#115267

Purpose

Expresses LbuCas13a from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

LbuCas13a

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-H-Ras_V12

Plasmid

#39504

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 (HRAS Human)

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pLHCX-FLAG-mPKM2

Plasmid

#44239

Depositor

Lewis Cantley, Matthew Vander Heiden

Article

Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

Insert

Pyruvate Kinase M2 (Pkm Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-HDAC6.ΔBuz-FLAG

Plasmid

#30484

Depositor

Tso-Pang Yao

Article

Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

Insert

HDAC6 (HDAC6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of the C-terminal Ubiquitin binding doma…

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pRST7-ER beta (long)

Plasmid

#11356

Depositor

Donald McDonnell

Article

Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

Insert

ER beta long (ESR2 Human)

Expression

Mammalian

Available Since

March 10, 2006

Availability

Academic Institutions and Nonprofits only
AAV-FLEX-Arch-GFP

Plasmid

#22222

Purpose

Cre-dependent viral expression of Arch-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Has Service

AAV1 and AAV9

Insert

Arch

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

no

Promoter

CAG

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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JN202: pMVP/SB/Blast-DEST

Plasmid

#121864

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-neo.ARRDC1-mCherry

Plasmid

#38319

Depositor

Quan Lu

Article

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

ARRDC1 (ARRDC1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pSB161 - pL2_pSB90_2x35S::GUS::tMAS

Plasmid

#123197

Purpose

binary plant vector for transient expression of GUS (with introns)

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::GUS::tMAS

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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