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Tus pGST-//

Plasmid

#53305

Purpose

Expresses GST-tagged E.coli replication terminator protein Tus.

Depositor

John Lis

Publication

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

E.Coli Tus replication terminator protein

Tags

GST

Expression

Bacterial

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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VVV

Plasmid

#27814

Depositor

Steven Vogel

Publication

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus-5-Venus-6-Venus

Use

Anisotropy and brightness standard

Expression

Mammalian

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pSMPUW-miR-124-GFP-Puro

Plasmid

#117321

Purpose

Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

Depositor

Volker Busskamp

Publication

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

hsa-miR-124-1 (MIR124-1 Human)

Use

Lentiviral

Tags

GFP-Puro

Expression

Mammalian

Promoter

EF1a

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MYO10-HMM-Nanotrap

Plasmid

#87255

Purpose

Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

Depositor

Thomas Friedman

Publication

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MYO10 (Myo10 Mouse)

Tags

GFP-nanotrap

Expression

Mammalian

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pQUAST-frt-stop-frt-p65AD::CaM

Plasmid

#64716

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Publication

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Inserts

p65AD::CaM
frt-stop-frt

Expression

Insect

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBS Wnt-4HA

Plasmid

#17375

Depositor

Jan Kitajewski

Publication

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-4HA (Wnt4 Mouse)

Tags

HA

Expression

Bacterial

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
mTFP1-Farnesyl-5

Plasmid

#55481

Purpose

Localization: Plasma Membrane, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Publication

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Farnesyl (HRAS Human)

Tags

mTFP1

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pDL025_HA-rb_IgG_HC_15F11

Plasmid

#193622

Purpose

HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.

Depositor

Jennifer DeLuca

Publication

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti_HA_15F11 rabbit heavy chain

Tags

signal peptide

Expression

Mammalian

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pETDuet-LahT150

Plasmid

#166785

Purpose

Cleavage of double glycine motif in peptides to remove the leader sequence

Depositor

Wilfred van der Donk

Publication

Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

Insert

LahT150

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-Positron-ST

Plasmid

#129254

Purpose

Express soma-localized Positron in mammalian cells

Depositor

Eric Schreiter

Publication

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Positron-ST

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pNOC-stacked-GFP-aequorin

Plasmid

#101011

Purpose

Construct for expression of a calcium sensitive luciferase reporter, GFP-aequorin, and hygromycin resistance gene by a bidirectional promoter

Depositor

Eva Farre

Publication

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pQUAST-frt-stop-frt-p65AD::CaM

Plasmid

#64716

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Publication

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Inserts

p65AD::CaM
frt-stop-frt

Expression

Insect

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBS Wnt-4HA

Plasmid

#17375

Depositor

Jan Kitajewski

Publication

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-4HA (Wnt4 Mouse)

Tags

HA

Expression

Bacterial

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-MYO10-HMM-Nanotrap

Plasmid

#87255

Purpose

Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

Depositor

Thomas Friedman

Publication

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MYO10 (Myo10 Mouse)

Tags

GFP-nanotrap

Expression

Mammalian

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3.1 Hmga2 tr

Plasmid

#14793

Depositor

David Bartel

Publication

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Hmga2 truncated (Hmga2 Mouse)

Expression

Mammalian

Mutation

truncataed HMGA2. As in human tumors, Hmga2-tr la…

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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PBLO CasX-gRNA

Plasmid

#126419

Purpose

E. coli vector for CasX gRNA --- Used for In Vitro Transcription

Depositor

Jennifer Doudna

Publication

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

casX gRNA

Use

CRISPR; In vitro transcription

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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His6_MBP_FMRP_P451S

Plasmid

#198693

Purpose

Expression of human FMRP protein in E. coli

Depositor

Simpson Joseph

Publication

Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

Insert

FMR1 (FMR1 Human)

Tags

MBP

Expression

Bacterial

Mutation

changed proline 451 to serine

Promoter

T7

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2 hBAK

Plasmid

#129579

Purpose

CRISPR deletion of human BAK

Depositor

Stephen Tait

Publication

Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

Insert

gRNA vs human BAK (BAK1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

None

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pNOC-stacked-GFP-aequorin

Plasmid

#101011

Purpose

Construct for expression of a calcium sensitive luciferase reporter, GFP-aequorin, and hygromycin resistance gene by a bidirectional promoter

Depositor

Eva Farre

Publication

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC22A1

Plasmid

#132034

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A1 (SLC22A1 Human)

Expression

Mammalian

Available since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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