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pcDNA3.2 mir-1-1 reporter hsa-mir-92a-1

Plasmid

#46672

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir-92a-1 (MIR92A1 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pAIP4_3H6Q

Plasmid

#234185

Purpose

Heterologous protein expression of macrocypin-1a in Escherichia coli

Depositor

Christopher Bahl

Insert

3H6Q

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pFd022.R2

Plasmid

#137967

Purpose

Expresses Prochlorococcus phage P-SSM2 phage Fd

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus phage P-SSM2 ferredoxin

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJTA022

Plasmid

#137975

Purpose

Inucible expression of Prochlorococcus phage P-SSM2 phage Fd for purification

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus phage P-SSM2 ferredoxin

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFd029.R2

Plasmid

#137968

Purpose

Expresses Prochlorococcus marinus NATL1A Fd 1

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus NATL1A ferredoxin 1

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFd030.R2

Plasmid

#137969

Purpose

Expresses Prochlorococcus marinus NATL2A Fd 1

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus NATL2A ferredoxin 1

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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A0A0A1ZY49

Plasmid

#163186

Purpose

Inducible expression of UniProt identifier A0A0A1ZY49

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0A1ZY49

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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A2CCV2

Plasmid

#163202

Purpose

Inducible expression of UniProt identifier A2CCV2

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A2CCV2

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pFd022.R2.RFP

Plasmid

#137971

Purpose

Expresses Prochlorococcus phage P-SSM2 phage Fd fused to mRFP

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus phage P-SSM2 ferredoxin fused to monomeric RFP

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFd029.R2.RFP

Plasmid

#137972

Purpose

Expresses Prochlorococcus marinus NATL1A Fd 1 fused to mRFP

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus NATL1A ferredoxin 1 fused to monomeric RFP

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFd030.R2.RFP

Plasmid

#137973

Purpose

Expresses Prochlorococcus marinus NATL2A Fd 1 fused to mRFP

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus NATL2A ferredoxin 1 fused to monomeric RFP

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFd031.RFP

Plasmid

#137974

Purpose

Expresses Prochlorococcus marinus MIT9211 Fd 1 fused to mRFP

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus MIT9211 ferredoxin 1 fused to monomeric RFP

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pNB_EA_R1CiM_4EGFP_R3EGFPi2

Plasmid

#185968

Purpose

Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Molecular IMPLY gate

Use

Synthetic Biology

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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p7SKU6SBpur

Plasmid

#160329

Purpose

dual sh RNA vector expressing cfSBFP2 and a puromycin selection marker

Depositor

Ikuo Wada

Article

Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

Type

Empty backbone

Tags

cfSBFP2

Expression

Mammalian

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSAC16

Plasmid

#137965

Purpose

Expresses Zea mays FNR and Prochlorococcus marinus NATL2A SIR

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Inserts

Zea mays Ferredoxin-NADP reductase
Procholoroccus marinus NATL2A sulfite reductase

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSAC17

Plasmid

#137966

Purpose

Expresses Zea mays FNR and Prochlorococcus marinus MIT9211 SIR

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Inserts

Zea mays Ferredoxin-NADP reductase
Procholoroccus marinus MIT9211 sulfite reductase

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pNB_EA_3EGFP

Plasmid

#185967

Purpose

Expresses EGFP from PR promoter in E.coli Dh5alpha cell

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Pr-Egfp cassette in forward direction in Network Brick

Use

Synthetic Biology

Promoter

PR

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pTOPO-rDNA-NTS

Plasmid

#23992

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

rDNA NTS

Use

Bacterial cloning - topo

Mutation

None

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pDONR221 Rrp8

Plasmid

#63775

Purpose

yeast ORF Rrp8 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Rrp8

Use

Gateway Cloning

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221 Spb1

Plasmid

#63777

Purpose

yeast ORF Spb1 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Spb1

Use

Gateway Cloning

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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