We narrowed to 12,121 results for: addgene/1000

1. GNRHR-Tango

   Plasmid

   #66297

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GNRHR (GNRHR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pLenti-5HRE-GFP NeoR

   Plasmid

   #128960

   Purpose

   Lentiviral plasmid for expression of hypoxia-responsive enhanced green fluorescent protein

   Depositor

   Carlos Carmona-Fontaine

   Article

   Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

   Insert

   5X HRE of VEGF (VEGFA Human)
   

   Use

   Lentiviral

   Tags

   d2EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV promoter

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PD2529 human beta1 full

   Plasmid

   #221401

   Purpose

   Mammalian expression of human integrin beta1 full-length

   Depositor

   Timothy Springer

   Article

   Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

   Insert

   integrin beta1 full-length (ITGB1 Human)
   

   Use

   Tags

   CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

   Expression

   Mammalian

   Mutation

   codon-optimized mature sequence

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFR (del3) L747-E749del, A750P

   Plasmid

   #11015

   Purpose

   Retroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR (del3) (EGFR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   L747-E749 deleted, A750P

   Promoter

   Available since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pg-ODD-EGFP

   Plasmid

   #87211

   Purpose

   HIF-1a's ODD domain fused to EGFP

   Depositor

   Violaine Sée

   Article

   Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

   Insert

   HIF-1a ODD domain (HIF1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   ODD domain (AA 530-652)

   Promoter

   CMV

   Available since

   March 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLS13-IL-10

   Plasmid

   #209128

   Purpose

   Transient mammalian expression of IL-10

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   IL-10 (IL10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. 8xNFAT-ZsG-muhCD4

   Plasmid

   #162745

   Purpose

   NFAT-driven ZsGreen-1 reporter gene with mutated human CD4 gene

   Depositor

   Maki Nakayama

   Article

   Landry et al Front Endocrinol (Lausanne). 2021 Mar 25

   Insert

   8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Human CD4 gene with glutamine to tyrosine at posi…

   Promoter

   Available since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-dCas13-inactive M3nls

   Plasmid

   #157854

   Purpose

   Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A; METTL3 D395A

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLDLR-Luc mutSRE

   Plasmid

   #14945

   Depositor

   Axel Nohturfft

   Article

   Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Insert

   LDLR promoter mut SRE (LDLR Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Point mutation in the SRE-1 sequence of the LDL r…

   Promoter

   Available since

   May 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-hAPOE-R-loop

    Plasmid

    #134901

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    APOE promoter (APOE Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZac2.1 GfaABC1D hM4D mCherry SV40

    Plasmid

    #92286

    Purpose

    AAV DREADD expression

    Depositor

    Baljit Khakh

    Article

    Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

    Insert

    hM4D (CHRM4 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    GfaABC1D

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-HA-LMW-FGF2

    Plasmid

    #157663

    Purpose

    Expresses FGF2 in mammalian cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-134

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. DRD1-Tango

    Plasmid

    #66268

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    DRD1 (DRD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ADCYAP1R1-Tango

    Plasmid

    #66208

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    ADCYAP1R1 (ADCYAP1R1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pBABE-hygro p53 DD

    Plasmid

    #9058

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    p53 DD (Trp53 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    DD. Dominant negative mutant carries deletion of…

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. TadA-NG-CBE

    Plasmid

    #227420

    Purpose

    C-to-T base editing in mammalian cells

    Depositor

    Yang Sun

    Article

    Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

    Insert

    TadA-nSpCas9-NG-2xUGI (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. miniSOG-ER-5

    Plasmid

    #57766

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    ER (CALR Human)
    

    Use

    Tags

    miniSOG

    Expression

    Mammalian

    Mutation

    ER Signal Peptide

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCMV-monSTIM1

    Plasmid

    #161795

    Purpose

    Mammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)

    Depositor

    Won Do Heo

    Article

    Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

    Insert

    MonSTIM1 (STIM1 Human, Mustard Weed)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-Rab11-DN (S25N)

    Plasmid

    #101046

    Purpose

    Expresses HA-tagged human Rab11-DN (S25N) in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    RAB11 (RAB11A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S25N

    Promoter

    CMV

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. DRD1-DuET

    Plasmid

    #213227

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    DRD1 (DRD1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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