We narrowed to 17,340 results for: emb

1. qTAG-N-Blast-V5

   Plasmid

   #207686

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-V5 cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. Flag TSC2 wt

   Plasmid

   #12132

   Depositor

   Cheryl Walker

   Article

   Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

   Insert

   TSC2 (TSC2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-SnoopTag-AffiHER2-SpyTag

   Plasmid

   #216280

   Purpose

   Expresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expression

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SnoopTag-AffiHER2-SpyTag
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6, SnoopTag, SpyTag, and Thrombin cleavage sit…

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TagBFP2- PIP4K2BD278K

   Plasmid

   #202729

   Purpose

   expresses fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   mTagBFP2:PIP4K2B (D278K) (PIP4K2B Human)
   

   Expression

   Mammalian

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP Tubulin K40A

   Plasmid

   #105304

   Purpose

   To study effects of acetylation-null tubulin

   Depositor

   Kenneth Yamada

   Article

   Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

   Insert

   alpha tubulin (TUBA1B Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K40A

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-N-Zeo-3xFLAG

   Plasmid

   #207706

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xFLAG cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. qTAG-N-Blast-3xHA

   Plasmid

   #207685

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xHA cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pMaCTag-Z31

   Plasmid

   #120074

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with V5

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   V5
   

   Use

   Pcr template

   Tags

   V5

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-moxGFP-Puro

   Plasmid

   #207721

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pGEX-4T1_4xUb

    Plasmid

    #199779

    Purpose

    Used for the expression of 4X human ubiquitin in tandem (SMC interal no. 538)

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    4X Ubiquitin in Tandem
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKL09-GCaMP6f-mScarlet bb118

    Plasmid

    #158979

    Purpose

    Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    GCaMP6f-mScarlet fusion
    

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#100833

    Promoter

    Bba_J23118 (sigma 70)

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR8-attR4-pegRNA-attR3

    Plasmid

    #213056

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    35S-CmYCLV-AtU6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCR8-attL5-pegRNA-attL4

    Plasmid

    #213055

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    35S-CmYCLV-AtU6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCR8-attL1-pegRNA-attR5

    Plasmid

    #213053

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    35S-CmYCLV-AtU6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-C-miniTurbo-Blast

    Plasmid

    #207714

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. qTAG-N-Puro-ultraID

    Plasmid

    #207693

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-ultraID cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pEF6.HA-ARRDC3

    Plasmid

    #38317

    Depositor

    Quan Lu

    Article

    Nabhan et al EMBO Rep. 2010 Aug;11(8):605-11. Epub 20

    Insert

    ARRDC3 (ARRDC3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-Rap1b

    Plasmid

    #118325

    Purpose

    Expression of bovine Rap1b

    Depositor

    Philip Stork

    Article

    Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

    Insert

    Rap1b (RAP1B Bovine)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRU48

    Plasmid

    #167670

    Purpose

    ENTRY vector for gRNA8 cloning under pAtU3 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU3
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMP116

    Plasmid

    #207918

    Purpose

    GreenBraid destination vector pGBZ1 - PaqCI - Eco31I - spisPink- Eco31I - PaqCI

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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