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pcry:GFP,-503unc:hACTA1GFP

Plasmid

#172529

Purpose

alphaA-crystallin promoter (cry) drives GFP expression in the lens. The 503bp unc-45b muscle-specific promoter drives expression of GFP-tagged human ACTA1.

Depositor

Joachim Berger

Publication

Berger et al Cell Rep. 2018 Jan 9;22(2):313-322. doi:

Insert

ATCA1

Expression

Mammalian

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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SNX9

Plasmid

#171943

Purpose

Lentiviral plasmid to express mouse SNX9 in mammalian cells

Depositor

Cheng-han Yu

Publication

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

SNX9

Use

Lentiviral

Expression

Mammalian

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRB33-GFP

Plasmid

#165483

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal uv-GFP, Flag and His

Depositor

Renato Bruni

Publication

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

10xHis-FLAG-GFP-TEV

Expression

Mammalian

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBTK205

Plasmid

#110587

Purpose

BTK Type 3 coding sequence for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP optim-1

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK599s

Plasmid

#110601

Purpose

BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP dropout

Use

Synthetic Biology

Available since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTEI121

Plasmid

#126061

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

GFP1-10

Use

Synthetic Biology

Tags

Linker (GTGGGGS)

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI122

Plasmid

#126062

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

ccdB Cassette

Use

Synthetic Biology; Module 0

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTK729

Plasmid

#114710

Purpose

Express NuMA(1-705+1700-2115ΔNLS)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-705+1700-2115ΔNLS)-RFP-Nano

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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BSP606

Plasmid

#122251

Purpose

expression vector for mECitrine under EFT3 promoter

Depositor

Alexander Mendenhall

Publication

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mECitrine

Expression

Worm

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTK388

Plasmid

#114713

Purpose

mAID-mClover-3FLAG tagging at DHC's C-terminal locus

Depositor

Tomomi Kiyomitsu

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

mAID-mClover-3FLAG

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT050d

Plasmid

#107901

Purpose

W2.5-2 plasmid. Contains PLacO-sgRNA1, PRha-sgRNA6-(sgRNA4-C16T-C18T) and is part of CAMERA 2.5

Depositor

David Liu

Publication

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PLacO-sgRNA1, PRha-sgRNA6-(sgRNA4-C16T-C18T)

Expression

Bacterial

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT046e

Plasmid

#107900

Purpose

W2.5-1 plasmid. Contains PTetO-SD8-BE2, PBAD-sgRNA5 (sgRNA3-A8C) and is part of CAMERA 2.5

Depositor

David Liu

Publication

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PBAD-sgRNA5 (sgRNA3-A8C)

Expression

Bacterial

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCG-A-celA(SP)-B

Plasmid

#87371

Purpose

An ORF part-containing vector harbouring the signal peptide from CelA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Publication

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

CelA signal peptide

Use

Synthetic Biology

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCG-A-xynA(SP)-B

Plasmid

#87372

Purpose

An ORF part-containing vector harbouring the signal peptide from XynA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Publication

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

XynA signal peptide

Use

Synthetic Biology

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-IA

Plasmid

#61671

Purpose

Non-fluorescent, truncated kinesin-1 fused to IA linker

Depositor

Kristen Verhey

Publication

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-IA (Kif5c Rat)

Expression

Mammalian

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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KIF5C(1-560)-EF(C)

Plasmid

#61675

Purpose

Non-fluorescent, truncated kinesin-1 fused to EF Hand linker

Depositor

Kristen Verhey

Publication

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-EF(C) (Kif5c Rat)

Expression

Mammalian

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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LI D-E 6xHIS

Plasmid

#54447

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

polyhistidine (6x) tag

Use

Cloning vector

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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LI C-D Cerulean c1

Plasmid

#54417

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

CFP Cerulean

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pMLLAC-J72041

Plasmid

#26186

Depositor

Christopher Anderson

Insert

Tev Protease Cleavage site

Use

Synthetic Biology; 2ab assembly vector

Available since

June 3, 2011

Availability

Academic Institutions and Nonprofits only

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pAVEC1_N-mScarletI

Plasmid

#231274

Purpose

For stably expressing fusion proteins with N terminally tagged mScarletI in plants

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mScarletI

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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