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pCS2+8-cyp-33e1-flag-tev-halo-his

Plasmid

#227151

Purpose

For T-REX experiments of cyp-33e1. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6-flag-tev-halo-his

Plasmid

#227154

Purpose

For T-REX experiments of CYP2A6. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLG-mLRRC45 (158 a.a.)

Plasmid

#240067

Purpose

ITT reporter plasmid for cold and osmotic stress

Depositor

Hodaka Fujii

Article

Maruyama et al Biol Methods Protoc. 2025 Jun 14;10(1):b

Insert

LRRC45 C-terminal 158 a.a. (Lrrc45 Mouse)

Use

Retroviral

Tags

LexA DNA-binding (DB) domain + Gal4 transactivati…

Expression

Mammalian

Promoter

LTR

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLG-mLRRC45 (209 a.a.)

Plasmid

#240068

Purpose

ITT reporter plasmid for cold stress

Depositor

Hodaka Fujii

Article

Maruyama et al Biol Methods Protoc. 2025 Jun 14;10(1):b

Insert

LRRC45 C-terminal 209 a.a. (Lrrc45 Mouse)

Use

Retroviral

Tags

LexA DNA-binding (DB) domain + Gal4 transactivati…

Expression

Mammalian

Promoter

LTR

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLG-mCCDC91 (198 a.a.)

Plasmid

#240069

Purpose

ITT reporter plasmid for cold stress

Depositor

Hodaka Fujii

Article

Maruyama et al Biol Methods Protoc. 2025 Jun 14;10(1):b

Insert

CCDC91 C-terminal 198 a.a. (Ccdc91 Mouse)

Use

Retroviral

Tags

LexA DNA-binding (DB) domain + Gal4 transactivati…

Expression

Mammalian

Promoter

LTR

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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TOPO-SV40

Plasmid

#226453

Purpose

For subcloning of SV40 promoter or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

SV40

Use

Cloning vector

Mutation

No

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ HA-BEX4

Plasmid

#231005

Purpose

Transient overexpression of human BEX4 in mammalian cells

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

BEX4 (BEX4 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP44

Plasmid

#196782

Purpose

Contains WRKY70 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

WRKY DNA-BINDING PROTEIN 70 (WRKY70 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP50

Plasmid

#196788

Purpose

Contains ACS2 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

1-AMINO-CYCLOPROPANE-1-CARBOXYLATE SYNTHASE 2 (ACS2 Mustard Weed)

Use

Greengate (gg) entry vector

Mutation

150 C->G

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP51

Plasmid

#196789

Purpose

Contains AOS promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

ALLENE OXIDE SYNTHASE (AOS Mustard Weed)

Use

Greengate (gg) entry vector

Mutation

2088 G->C ; 1434 G->C

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP55

Plasmid

#196793

Purpose

Contains PDF1.2 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

PLANT DEFENSIN 1.2 (PDF1.2 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP56

Plasmid

#196794

Purpose

Contains PR1 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

PATHOGENESIS-RELATED GENE 1 (PR1 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP59

Plasmid

#196797

Purpose

Contains MYB72 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

ARABIDOPSIS THALIANA MYB DOMAIN PROTEIN 72 (MYB72 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP27

Plasmid

#196765

Purpose

Contains CPK5 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

CALMODULIN-DOMAIN PROTEIN KINASE 5 (CPK5 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP30

Plasmid

#196768

Purpose

Contains GLR2.7 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

GLUTAMATE RECEPTOR 2.7 (GLR2.7 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP35

Plasmid

#196773

Purpose

Contains RCD1 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

RADICAL-INDUCED CELL DEATH1 (RCD1 Mustard Weed)

Use

Greengate (gg) entry vector

Mutation

248 G->C

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP41

Plasmid

#196779

Purpose

Contains WRKY33 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

WRKY DNA-BINDING PROTEIN 33 (WRKY33 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP14

Plasmid

#196752

Purpose

Contains LYM1 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

LYSM DOMAIN GPI-ANCHORED PROTEIN 1 PRECURSOR (LYM1 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET41SIII-ATF6(1-373)

Plasmid

#171074

Purpose

Expresses of ATF6α(1-373) in bacteria

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

ATF6α (ATF6 Human)

Tags

GST and His

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-UDP1.0-WPRE

Plasmid

#220849

Purpose

AAV virus packaging plasmid for neuronal expression of an extracellular UDP sensor (UDP1.0)

Depositor

Yulong Li

Article

Umpierre et al Neuron. 2024 Apr 10:S0896-6273(24)00195-

Insert

UDP1.0

Use

AAV

Promoter

human Synapsin (hSyn)

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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