We narrowed to 23,381 results for: puromycin

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pMXs-EGFP-Rheb-IP

Plasmid

#13831

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

Rheb (Rheb Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pAPM-D4 miR30-TRIM28 ts3

Plasmid

#115864

Purpose

TRIM28 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

TRIM28 shRNA (TRIM28 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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betaTrCP mVenus mutant reporter

Plasmid

#192459

Purpose

Fluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.

Depositor

Steven Cappell

Article

Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

Insert

CdC25B

Use

Lentiviral

Tags

SV40 NLS and mVenus

Expression

Mammalian

Mutation

aa198-338 of CDC25B. betaTrCP degron sequence (aa…

Promoter

EF1alpha

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor

Plasmid

#12153

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor (RHOA Human)

Use

Retroviral

Tags

6XHis

Expression

Mammalian

Available Since

July 7, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-GpNLuc

Plasmid

#208840

Purpose

Expresses FLAG epitope-tagged GpNLuc "Green" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

GpNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR

Plasmid

#206213

Purpose

PiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplification

Depositor

Masamichi Kamihira

Article

Kitano et al Cytotechnology. 2021 Jun;73(3):353-362.

Insert

tetracycline-responsive transactivator

Expression

Mammalian

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-CeNL

Plasmid

#208839

Purpose

Expresses FLAG epitope-tagged CeNL "Blue" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

CeNL Nanolantern

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_ALDH7A1

Plasmid

#106120

Purpose

Lentiviral expression of ALDH7A1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

ALDH7A1 (ALDH7A1 Human)

Use

Lentiviral

Tags

DDK (FLAG) and Myc

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT PUR mGSK3AWT

Plasmid

#35159

Depositor

Jim Woodgett

Article

Doble et al Dev Cell. 2007 Jun;12(6):957-71.

Insert

GSK-3α (Gsk3a Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2012

Availability

Industry, Academic Institutions, and Nonprofits
LC201: pMVP/PB/Puro-DEST

Plasmid

#121874

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LPC-flag-REST-FS

Plasmid

#41904

Depositor

Stephen Elledge

Article

Westbrook et al Nature. 2008 Mar 20;452(7185):370-4. doi

Insert

RE1-silencing transcription factor (REST Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

FS mutant

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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Nanog-2A-GFP

Plasmid

#59994

Purpose

Inserts GFP immediately downstream of the Nanog coding region

Depositor

Rudolf Jaenisch

Article

Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

Insert

Nanog (Nanog Mouse)

Use

Mouse Targeting

Tags

2A-GFP

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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H2B-2A-GFP-IRESpuro2

Plasmid

#183746

Purpose

Expresses H2B-2A-GFP

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

H2B (H2BC16P Human)

Tags

GFP with 2A protease cleavage site between H2B an…

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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attB-puro-GA donor

Plasmid

#182138

Purpose

attB-puro-GA DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attB-GA-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-puro PM-mKate2-P2A-GEM-LTB4

Plasmid

#202643

Purpose

Expresses the LTB4 sensor GEM-LTB4 along with the plasma membrane targeted mKate2 tag in mammalian cells

Depositor

Balázs Enyedi

Article

Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

Insert

PM-mKate2-P2A-GEM-LTB4

Tags

PM-mKate2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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sgLenti

Plasmid

#105996

Purpose

single sgRNA expression vector

Depositor

Mike McManus

Article

Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

Type

Empty backbone

Use

CRISPR and Lentiviral; S.pyogenes sgrna expressio…

Expression

Mammalian

Promoter

U6

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Myc-Oct3/4-IP

Plasmid

#13460

Depositor

Shinya Yamanaka

Article

Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

Insert

Oct3/4 (Pou5f1 Mouse)

Tags

Myc (6x)

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pSLQ-5S-F30-Control

Plasmid

#127582

Purpose

Expresses 5S-F30-Control rRNA from a mU6 promoter

Depositor

David Rueda

Article

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

5S rRNA with F30 folding scaffold

Expression

Mammalian

Promoter

modified U6

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-SIRT6-H133Y

Plasmid

#102327

Purpose

Human SIRT6 H133Y for mammalian expression

Depositor

Hening Lin

Article

Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

Insert

SIRT6 (SIRT6 Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

changed histidine 133 to tyrosine

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDECKO_TFRC_C (with mCherry)

Plasmid

#72627

Purpose

Expresses two gRNAs targeting the TFRC promoter

Depositor

Roderic Guigo, Rory Johnson

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward TFRC

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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