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pcDNA3.1-TFEBS122D-MYC (#777)

Plasmid

#99956

Purpose

mammalian expression

Depositor

James Brugarolas

Publication

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Insert

TFEB (TFEB Human)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

S122D

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LentiGuide-puro-NLS-GFP

Plasmid

#185473

Purpose

Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

Depositor

Daniel Durocher

Publication

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

5 Articles

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pFA6a-HaloTag-KanMX6

Plasmid

#87029

Purpose

Tag S. pombe genes in C terminal with HaloTag

Depositor

Julien Berro

Insert

HaloTag

Expression

Bacterial and Yeast

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMXs-mGCN2-FLAG-IP

Plasmid

#101794

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse GCN2

Depositor

Seiichi Oyadomari

Publication

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Gcn2 (Eif2ak4 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGWB414

Plasmid

#74808

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB553

Plasmid

#74883

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDNA-FLAG-METTL3-APPA

Plasmid

#160251

Purpose

Express catalytic inactive FLAG-METTL3 in human cells

Depositor

Alessandro Fatica

Publication

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changes in aa 395–398, DPPW → APPA

Promoter

CMV

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMSCV-puro-mycRSK2wt

Plasmid

#15827

Depositor

Jing Chen

Publication

Kang et al Cancer Cell. 2007 Sep . 12(3):201-14.

Insert

Ribosomal S6 kinase 2 (Rps6ka3 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

wild type

Available since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Beclin1 1-242

Plasmid

#24391

Depositor

Qing Zhong

Publication

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains amino acids 1-242.

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pBSFI-myr-akt-T308D/S473D

Plasmid

#49192

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from T to D at position 308 and S to D at position 473 in cloning vector

Depositor

Peter Vogt

Publication

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Mutation

T308D; S473D

Promoter

T7

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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p426 25Q GPD

Plasmid

#1181

Depositor

Susan Lindquist

Publication

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 25Q (HTT Human)

Tags

GFP

Expression

Yeast

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pLenti-EF1a-Backbone(NI)

Plasmid

#27962

Depositor

Feng Zhang

Publication

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-Backbone(NI)

Use

Lentiviral

Expression

Mammalian

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lenti-SpCas9 puro

Plasmid

#104994

Purpose

This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAGM8031

Plasmid

#48037

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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pICH41766

Plasmid

#48018

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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CMV-T7-hOsa1

Plasmid

#17986

Depositor

Naoko Tanese

Publication

Inoue et al J Biol Chem. 2002 Nov 1. 277(44):41674-8

Insert

Osa1 (ARID1A Human)

Tags

T7 epitope

Expression

Mammalian

Mutation

Missing 5' end

Available since

Aug. 19, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
Lenti-human-UBDUB-gRNA-puromycin-IRES-CFP

Pooled library

#171531

Purpose

Knockout library targeting ubiquitination and de-ubiquitination complex genes.

Depositor

Chunliang Li

Publication

Wright et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Expression

Mammalian

Species

Homo sapiens

Use

Lentiviral

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pB-CAGGS-dCas9

Plasmid

#110823

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, George Church

Publication

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pShuttle-Flag-XBP1u

Plasmid

#63679

Purpose

Adenoviral shuttle vector for XBP1u expression

Depositor

Qingbo Xu, Lingfang Zeng

Publication

Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

Insert

XBP1 (XBP1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

unspliced

Promoter

CMV

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
SGEP-sh-Hs-PHGDH-1956

Plasmid

#188670

Purpose

shRNA

Depositor

Lewis Cantley

Insert

shPHGDH (PHGDH Human)

Use

CRISPR and Lentiviral

Mutation

WT

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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