We narrowed to 19,002 results for: Tec

1. pT7-ZoAl-END-3xF-ORF

   Plasmid

   #238485

   Purpose

   For production of C-term flag-tagged ZoAl EN-dead ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Mutation

   DD1041/1054->AA

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-TaGu-RTD-3xF-ORF

   Plasmid

   #238490

   Purpose

   For production of C-term flag-tagged TaGu RT-dead ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Mutation

   DD660/661->AA

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-TaGu-END-3xF-ORF

   Plasmid

   #238489

   Purpose

   For production of C-term flag-tagged TaGu EN-dead ORF mRNA for mRNA transfection in PRINT

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement mRNA
   

   Use

   Ivt template

   Tags

   3x-Flag

   Mutation

   DD1057/1070->AA

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSNRW-Z5-Nluc

   Plasmid

   #174487

   Purpose

   Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

   Depositor

   Richard C. Willson

   Article

   Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

   Insert

   Z5-Nluc
   

   Tags

   6x Histidine tag (N terminal on insert)

   Expression

   Bacterial

   Promoter

   T7lac promoter

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTLR repair vector

   Plasmid

   #64322

   Purpose

   Vector for repair of the traffic light reporter (TLR), providing the venus coding region

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Insert

   Venus
   

   Use

   Artificial targeting vector

   Mutation

   see publication for details

   Promoter

   none

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2-HA-GAAZFP-FokI-RR

   Plasmid

   #18754

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

   Insert

   zinc finger nuclease
   

   Expression

   Bacterial

   Mutation

   RNA expression vector for expressing the zinc fin…

   Available Since

   July 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115220

   Purpose

   Expresses Pin3Cas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   Pin3Cas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115210

   Purpose

   Expresses AspCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   AspCas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115214

   Purpose

   Expresses PauCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PauCas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB445

    Plasmid

    #74831

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. SQT1665

    Plasmid

    #78744

    Purpose

    Human expression plasmid for LbCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB518

    Plasmid

    #74860

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 4xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGWB550

    Plasmid

    #74880

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19/Mito-mChe

    Plasmid

    #183167

    Purpose

    Construct for transient expression of mitochondrion-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    Mito-mCherry
    

    Tags

    OsCOX11, Oryza sativa cytochrome c oxidase assemb…

    Expression

    Plant

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQC.Flip-nluc(LgBiT1-8)CP[Casp3A]-Fluc

    Plasmid

    #200123

    Purpose

    Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A] protease reporter and P2A firefly luciferase in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[Casp3A] P2A firefly luciferase
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

    Plasmid

    #92390

    Purpose

    expresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cells

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. eNme2-C TadCBEd

    Plasmid

    #193841

    Purpose

    Express TadCBEd (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    eNme2-C TadCBEd
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRDB_156

    Plasmid

    #216090

    Purpose

    Cas9 [Sp] CRISPRa targeting CD274, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD274 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. SQT1659

    Plasmid

    #78743

    Purpose

    Human expression plasmid for AsCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAF0688 Kp03_attP-mCherry donor

    Plasmid

    #193467

    Purpose

    Promoterless mcherry donor for Kp03

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03_attP
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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