We narrowed to 32,650 results for: Eng

1. CMV-CASANOVA

   Plasmid

   #113035

   Purpose

   CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-EF1a-FLEX-SOUL-P2A-tdTomato-WPRE-BGHpA

   Plasmid

   #177577

   Purpose

   Expresses SOUL in Cre-positive cells

   Depositor

   Guoping Feng

   Article

   Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

   Insert

   SOUL
   

   Use

   AAV

   Tags

   tdTomato

   Promoter

   EF1a

   Available Since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGD-P1/HC-Pro

   Plasmid

   #196328

   Purpose

   35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressor

   Depositor

   Zhenghe Li

   Article

   Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

   Insert

   tobacco etch virus P1/HC-Pro
   

   Expression

   Plant

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. sg_Trp53_i3-ipUSEPR-TR657

   Plasmid

   #228929

   Purpose

   Knockdown of Trp53 in mammalian cells

   Depositor

   Sika Zheng

   Article

   Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

   Insert

   sg_Trp53_i3 (Trp53 Mouse)
   

   Use

   Lentiviral

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TRE-Lepr_FL-IRES-eGFP

   Plasmid

   #196635

   Purpose

   Doxycycline inducible expression of full length mouse Lepr with eGFP marker

   Depositor

   Elaine Fuchs

   Article

   Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

   Insert

   Lepr (Lepr Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA 3.3 N-MYC RMDN3

   Plasmid

   #227878

   Purpose

   Used for transfection of cells for expression of C-MYC RMDN3

   Depositor

   Sheng-cai Lin

   Article

   Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

   Insert

   RMDN3 (RMDN3 Human)
   

   Tags

   MYC

   Expression

   Mammalian

   Available Since

   July 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-EF1a-fDIO-Cre

   Plasmid

   #121675

   Purpose

   Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

   Depositor

   Esteban Engel, Alexander Nectow

   Article

   Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

   Has Service

   AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

   Insert

   NLS-CRE-HA
   

   Use

   AAV

   Tags

   3xHA and SV40-NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EBFP2-UtrCH

   Plasmid

   #124656

   Purpose

   F-actin marker. EBFP2 is fused to the calponin homology domain of utrophin (UtrCH).

   Depositor

   Cheng-han Yu

   Article

   Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

   Insert

   Utrophin (UTRN Human)
   

   Tags

   EBFP2

   Expression

   Mammalian

   Mutation

   see depositor comments below

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-hSyn-SOUL-P2A-TdTomato-WPRE-hGH-polyA

   Plasmid

   #177576

   Purpose

   Expresses SOUL in neuronal cell types

   Depositor

   Guoping Feng

   Article

   Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

   Insert

   SOUL
   

   Use

   AAV

   Tags

   tdTomato

   Promoter

   hSyn

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Tol2-BetaActin-SecA5-YFP

    Plasmid

    #105664

    Purpose

    Ubiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporter

    Depositor

    Qing Deng

    Article

    Hsu et al Front Immunol. 2018 Mar 29;9:607. doi: 1

    Insert

    Secreted Annexin5-EYFP (ANXA5 Human)
    

    Use

    Tol2-gateway

    Tags

    EYFP

    Promoter

    Beta-Actin

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPbcas13b

    Plasmid

    #89906

    Purpose

    Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    Cas13b
    

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ABE7.10-F148A

    Plasmid

    #132946

    Purpose

    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

    Depositor

    Hui Yang

    Article

    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

    Insert

    ABE7.10(F148A)
    

    Use

    CRISPR; Base editor

    Expression

    Mammalian

    Mutation

    ABE7.10(F148A)

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Clone 16 - 2 HH06 pFUSE-IgG1-Knob-mutation knob-clone6

    Plasmid

    #192184

    Purpose

    Clone 16 (HH06) pFUSE-IgG1-Knob-mutation knob-clone6

    Depositor

    Sidi Chen

    Article

    Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

    Insert

    Clone 6 heavy chain (HH06)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Clone 16 - 1 HH02 pFUSE-IgG1-Hole mutation-hole-clone2

    Plasmid

    #192183

    Purpose

    Clone 16 (HH02) pFUSE-IgG1-Hole mutation-hole-clone2

    Depositor

    Sidi Chen

    Article

    Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

    Insert

    Clone 2 heavy chain (HH02)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ACE2

    Plasmid

    #164219

    Purpose

    Expresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection marker

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Mutation

    silent mutation to remove internal EcoRI site (GA…

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHIV-INK4A-zsGreen

    Plasmid

    #110728

    Purpose

    Mammalian Expression of p16INK4A IRES zsGreen

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    p16INK4A IRES zsGreen (CDKN2A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV pCAG-mRuby3-WPRE

    Plasmid

    #107744

    Purpose

    Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

    Depositor

    Rylan Larsen

    Article

    Zhuang et al Cell Rep. 2021 Oct 12;37(2):109826. doi:

    Insert

    mRuby3
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGES401

    Plasmid

    #190198

    Purpose

    For CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selection

    Depositor

    Yuefeng Guan

    Article

    Bai et al Mol Plant. 2022 Jul 4;15(7):1081-1083. d

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    3xFlag

    Expression

    Plant

    Mutation

    plant-codon optimized

    Promoter

    Glycine max elongation factor 1A(pM4)

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-Flag-mRIPK1-S321A

    Plasmid

    #115339

    Purpose

    Mammalian expression of Flag-mRIPK1-S321A

    Depositor

    Junying Yuan

    Article

    Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

    Insert

    RIPK1 (Ripk1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S321A

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MBP-FUS_FL_12E

    Plasmid

    #98652

    Purpose

    Expresses MBP-tagged full length phosphomimetic FUS

    Depositor

    Nicolas Fawzi

    Article

    Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

    Insert

    FUS (FUS Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    12 S/T to E

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge