We narrowed to 30,912 results for: LIS

1. pLenti_dNmeCas9

   Plasmid

   #120077

   Purpose

   dNmeCas9 with no sgRNA cassette lenti construct

   Depositor

   Erik Sontheimer

   Article

   Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

   Insert

   dNmeCas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaG-8

   Plasmid

   #172761

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-8
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-pelB-LaG-5

   Plasmid

   #172760

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-5
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZJQIBEBT003

   Plasmid

   #140053

   Purpose

   The pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.

   Depositor

   Jia Zhang

   Article

   Zhang et al ACS Synth Biol. 2020 Apr 17;9(4):940-952

   Insert

   eMutS-L157C-G233C-CBM-EGFP
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   L157C, G233C

   Promoter

   T7

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-UBE3B-BirA-Puro

   Plasmid

   #107352

   Purpose

   Expresses UBE3B fused to BirA in mammalian cells.

   Depositor

   Robert Sobol

   Article

   Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

   Insert

   UBE3B-BirA (UBE3B Human)
   

   Use

   Lentiviral

   Tags

   Biotin ligase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 23, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pGL4.24-Cd36-enhancer5

   Plasmid

   #138577

   Purpose

   Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17893352_17894696

   Depositor

   Lora Hooper

   Article

   Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

   Insert

   Cd36 Promoter (Cd36 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Minimal promoter

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cs2+Etv5

   Plasmid

   #49095

   Purpose

   expression of Etv5

   Depositor

   Michael Tsang

   Article

   Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

   Insert

   Etv5 (etv5a Zebrafish)
   

   Use

   Xenopus/avian/zebrafish

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAWp100

   Plasmid

   #157977

   Purpose

   pBT264-mutmU6p-{2xBbsI}-sgRNA scaffold-{MfeI}

   Depositor

   Alan Siu Lun Wong

   Article

   Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmCherry-3xNLS-OptoJNKi

   Plasmid

   #89740

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFUW-TetO-Txnip

    Plasmid

    #178437

    Purpose

    doxycycline-inducible expression of Mouse Txnip in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    Txnip (Txnip Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS-tRNA-Ser

    Plasmid

    #174318

    Purpose

    For in vitro transcription of mouse tRNAs

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    tRNA-ser
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Myc-delta

    Plasmid

    #118037

    Purpose

    GABAA receptor expression (delta subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Article

    Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

    Insert

    GABRD (Gabrd Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCVpic2neo-hTERT-FF2

    Plasmid

    #164910

    Purpose

    Expresses hTERT cDNA and FF2 shRNA in mammalian cells

    Depositor

    Ji Luo

    Article

    Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

    Inserts

    human telomerase reverse transcriptase (TERT Human)
    Neuropeptide FF receptor 2 (NPFFR2 Human)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEBG-MKK4-K129R (GST)

    Plasmid

    #21564

    Depositor

    Leonard Zon

    Article

    Sanchez et al Nature. . 372(6508):794-8.

    Insert

    mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Lysine 129 to arginine

    Promoter

    Available since

    July 17, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pKCAG-mCherry-OptoJNKi

    Plasmid

    #89738

    Purpose

    Expression of light-regulated JNK inhibitor, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    OptoJNKi
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-JNKsub(TA)DD-YPet-NES

    Plasmid

    #84634

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    JNKsub(TA)DD-YPet-NES
    

    Use

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-M2-TLR2-V660N

    Plasmid

    #12292

    Depositor

    Bruce Beutler

    Article

    Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    TLR2 (Tlr2 Mouse)
    

    Use

    Tags

    FLAG (FLAG-M2)

    Expression

    Mammalian

    Mutation

    M82I, H202Y and V660N; signal peptide (aa1-23) re…

    Promoter

    Available since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pVL94 - pOp4:VND7

    Plasmid

    #116016

    Purpose

    destination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-induction

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmCherry-NES-OptoJNKi

    Plasmid

    #89739

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-M2-TLR2-P681H

    Plasmid

    #12290

    Depositor

    Bruce Beutler

    Article

    Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    TLR2 (Tlr2 Mouse)
    

    Use

    Tags

    FLAG (FLAG-M2)

    Expression

    Mammalian

    Mutation

    A74V, M82I, H202Y and P681H; signal peptide (aa1-…

    Promoter

    Available since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only