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SP_H6_Halo_WT_KDEL_pBABEpu

Plasmid

#183688

Purpose

Retroviral expression of ER targeted H6-tagged Halotag Wild type

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo

Use

Retroviral

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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HySp5-310

Plasmid

#192995

Purpose

Plasmid that encodes for Hydra Sp5, lacking the SP box

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Tags

HA

Expression

Mammalian

Mutation

no SP box

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGEM-T-Easy-HySp5-557

Plasmid

#193000

Purpose

Plasmid to generate an ISH probe for Hydra Sp5

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Use

Cloning vector

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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HySp5:Luc

Plasmid

#192985

Purpose

Plasmid to test the Hydra Sp5 promoter activity

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5 promoter

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ZfSp5l1

Plasmid

#192999

Purpose

Plasmid that encodes for Zebrafish Sp5-like

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Zebrafish Sp5-like

Tags

HA

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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HySp5-337

Plasmid

#192994

Purpose

Plasmid that encodes for Hydra Sp5, lacking the DNA binding domain

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Tags

HA

Expression

Mammalian

Mutation

no DNA binding domain

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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ZfWnt3:Luc

Plasmid

#192992

Purpose

Plasmid to test the Zebrafish Wnt3 promoter activity

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Zebrafish Wnt3 promoter

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α-mouse-CHMP3(147)-HA

Plasmid

#154177

Purpose

expresses mouse CHMP3(147) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of CHMP3 at amino acid 147

Promoter

EF1alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-squirrel-monkey-CHMP3(155)

Plasmid

#154183

Purpose

expresses squirrel monkey CHMP3(155) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3(155)

Tags

FLAG, Strep-Tag II, and preScission site

Expression

Mammalian

Mutation

C-terminal truncation of CHMP3 at amino acid 155

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCdkn3

Plasmid

#160955

Purpose

Cdkn3 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdkn3 shRNA (Cdkn3 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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CC1-Pos6

Plasmid

#101656

Purpose

CC1-Pos6 is a modified version of CC1-M240 that has 4 arginines in its linker. (* super ecliptic pHluorin A227D)

Depositor

Bradley Baker

Article

Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

Insert

CC1-Pos6

Expression

Mammalian

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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shRNA_ZNF652_1

Plasmid

#215202

Purpose

Supression of shZNF652 expression

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

ZNF652 shRNA 1 (ZNF652 Human)

Use

Lentiviral

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-1_NbATML1-2pro and NbATML1-1pro

Plasmid

#231154

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLP4

Plasmid

#209959

Purpose

Entry vector to store Level 0* parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLP5

Plasmid

#209960

Purpose

Entry vector to store Level 0* resistance cassette parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLP6

Plasmid

#209961

Purpose

Entry vector to store Level 0* replication origin parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SP_H6_Halo_L172Q_KDEL_pBABEpu

Plasmid

#183690

Purpose

Retroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloL172Q)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(L172Q)

Use

Retroviral

Mutation

L72Q in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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SP_H6_Halo_M21K_F86L_KDEL_pBABEpu

Plasmid

#183691

Purpose

Retroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloM21K_F86L)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(M21K_F86L)

Use

Retroviral

Mutation

M21K_F86L in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_CHO-Bip_gRNA1

Plasmid

#183694

Purpose

CRISPRa gRNA expression vector for Chinese Hamster Bip (Hspa5)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

gRNA targeting chinese hamster Bip (Hspa5)

Use

CRISPR

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET30_Halotag_K73T_Jdomain

Plasmid

#183693

Purpose

Bacterial expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) fused with J domain

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(K73T)-J domain

Expression

Bacterial

Mutation

K73T in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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