Addgene

We narrowed to 338 results for: ACL

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hGli3 NTOTAL in AINGpCAGGS

Plasmid

#121973

Purpose

medium Gli3 transcriptional repressor

Depositor

James Briscoe

Article

Ulloa et al Curr Biol. 2007 Mar 20;17(6):545-50. doi

Insert

hGli3 NTOTAL (GLI3 Human)

Use

Chick electroporation

Tags

5xmyc and Linker A IRES NLS GFP pCAGGS

Expression

Mammalian

Mutation

C terminus deletion from zinc finger domain

Promoter

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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DN2-Gli3 in AINGpCAGGS

Plasmid

#121969

Purpose

strong Gli3 transcriptional activator

Depositor

James Briscoe

Article

Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

Insert

DN2Gli3 (GLI3 Human)

Use

Chick electroporation

Tags

5xmyc

Expression

Mammalian

Mutation

N terminus deletion (nt1-1401)

Promoter

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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DN1-Gli3 in AINGpCAGGS

Plasmid

#121970

Purpose

medium Gli3 transcriptional activator

Depositor

James Briscoe

Article

Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

Insert

DN2Gli3 (GLI3 Human)

Use

Chick electroporation

Tags

5xmyc

Expression

Mammalian

Mutation

N terminus deletion (nt1-864)

Promoter

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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NRCoC

Plasmid

#74300

Purpose

Mammalian expression of full-length treacle fused to GFP

Depositor

Rita Shiang

Article

Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

Insert

treacle ribosome biogenesis factor 1 (Tcof1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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CΔ1

Plasmid

#74296

Purpose

Mammalian expression of treacle (residues 1176-1302) fused to GFP

Depositor

Rita Shiang

Article

Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

Insert

treacle ribosome biogenesis factor 1 (Tcof1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Expresses residues 1176 - 1302

Promoter

CMV

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
CΔ4

Plasmid

#74299

Purpose

Mammalian expression of treacle (residues 1271-1302) fused to GFP

Depositor

Rita Shiang

Article

Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

Insert

treacle ribosome biogenesis factor 1 (Tcof1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Expresses residues 1271 - 1302

Promoter

CMV

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
CΔ2

Plasmid

#74297

Purpose

Mammalian expression of treacle (residues 1176-1270) fused to GFP

Depositor

Rita Shiang

Article

Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

Insert

treacle ribosome biogenesis factor 1 (Tcof1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Expresses residues 1176 - 1270

Promoter

CMV

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only
CΔ3

Plasmid

#74298

Purpose

Mammalian expression of treacle (residues 1262-1302) fused to GFP

Depositor

Rita Shiang

Article

Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

Insert

treacle ribosome biogenesis factor 1 (Tcof1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Expresses residues 1262 - 1302

Promoter

CMV

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
cASIC1 concatemer - vector backbone

Plasmid

#233132

Purpose

To create cASIC1 channels of defined stoichiometry in mammalian cells

Depositor

David MacLean

Article

Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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Rabbit SERCA2b (pBSII-SK-)

Plasmid

#75186

Purpose

Cloning vector for ATP2A2

Depositor

David MacLennan, Jonathan Lytton

Article

Lytton et al J Biol Chem. 1989 Apr 25;264(12):7059-65

Insert

ATP2A2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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VV001: WT Arch-3 in pET28b

Plasmid

#58487

Purpose

Expresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coli

Depositor

Adam Cohen

Article

Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

Insert

Archaerhodopsin-3 (with C-terminal His tag)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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VV020: WT Arch-3 - eGFP in pET28b

Plasmid

#58488

Purpose

Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coli

Depositor

Adam Cohen

Article

Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

Insert

Archaerhodopsin-3

Use

Tags

eGFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Human SERCA3 (pMT2)

Plasmid

#75189

Purpose

Mammalian expression of ATP2A3

Depositor

David MacLennan, Jonathan Lytton

Article

Poch et al Am J Physiol. 1998 Dec;275(6 Pt 1):C1449

Insert

ATP2A3 (ATP2A3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

adenovirus major late promoter

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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cASIC1 concatemer - fully assembled

Plasmid

#233133

Purpose

To create cASIC1 channels of defined stoichiometry in mammalian cells

Depositor

David MacLean

Article

Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

Insert

ASIC1 (ASIC1 Chicken)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
cASIC1 concatemer - Subunit C

Plasmid

#233131

Purpose

To create cASIC1 channels of defined stoichiometry in mammalian cells

Depositor

David MacLean

Article

Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

Insert

ASIC1 (ASIC1 Chicken)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
cASIC1 concatemer - Subunit B

Plasmid

#233130

Purpose

To create cASIC1 channels of defined stoichiometry in mammalian cells

Depositor

David MacLean

Article

Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

Insert

ASIC1 (ASIC1 Chicken)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
cASIC1 concatemer - Subunit A

Plasmid

#233129

Purpose

To create cASIC1 channels of defined stoichiometry in mammalian cells

Depositor

David MacLean

Article

Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

Insert

ASIC1 (ASIC1 Chicken)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-ZsGreen-P2A

Plasmid

#222648

Purpose

Vector for cloning gene of interest for in vitro transcription

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

ZsGreen

Use

In vitro transcription

Tags

Expression

Mutation

mutated stop codon prior to a P2A peptide

Promoter

T7

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Human SERCA2b (pcDNA3.1+)

Plasmid

#75188

Purpose

Mammalian expression of ATP2A2

Depositor

David MacLennan, Jonathan Lytton

Article

Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

Insert

ATP2A2 (ATP2A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Human SERCA2a (pcDNA3.1+)

Plasmid

#75187

Purpose

Mammalian expression of ATP2A2

Depositor

David MacLennan, Jonathan Lytton

Article

Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

Insert

ATP2A2 (ATP2A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge