We narrowed to 31,499 results for: LIS;

1. pCAG-Cas12i2-T2A-EGFP

   Plasmid

   #188270

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Chen et al Innovation (Camb). 2022 May 26;3(4):1002

   Insert

   Cas12i2
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lentiCRISPR_DKO

   Plasmid

   #183192

   Purpose

   Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

   Depositor

   Lang Li

   Article

   Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6, mU6

   Available Since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-hsyn-GRAB_ATP1.0

   Plasmid

   #167577

   Purpose

   Express the ATP sensor GRAB_ATP1.0 in neurons

   Depositor

   Yulong Li

   Article

   Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

   Insert

   ATP sensor GRAB_ATP1.0 (P2RY1 Human)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAVS1-MAFB-IRF8-CEBPA

   Plasmid

   #190720

   Purpose

   For microglia differentiation from iPSC

   Depositor

   Li Gan

   Article

   Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

   Insert

   MAFB-IRF8-CEBPA
   

   Expression

   Mammalian

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-hSyn-GRAB_g5-HT3.0mut

   Plasmid

   #208718

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. iCAP-ROOT

   Plasmid

   #192262

   Purpose

   AAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsid

   Depositor

   Li Ye

   Article

   Wang et al Nature. 2022 Sep;609(7927):569-574. doi:

   Insert

   Synthetic construct for ROOT VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pM-CBE

   Plasmid

   #196293

   Purpose

   35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV M antigenome encoding CBE in place of viral GP
   

   Use

   CRISPR

   Tags

   3×flag

   Expression

   Plant

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pM-ABE

   Plasmid

   #196292

   Purpose

   35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV M antigenome encoding ABE in place of viral GP
   

   Use

   CRISPR

   Tags

   3×flag

   Expression

   Plant

   Mutation

   See depositor comments below

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ePE6d

   Plasmid

   #229029

   Purpose

   Expresses ePE6d — engineered SpCas9 nickase (nCas9), RNA chaperone nucleocapsid (NC) protein, with T128N/N200C/V223Y variant of MMLVΔRNaseH — for gene editing in rice

   Depositor

   Li Juan

   Article

   Xu et al Plant Biotechnol J. 2024 Sep 27. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Feb. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGD-P1/HC-Pro

    Plasmid

    #196328

    Purpose

    35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressor

    Depositor

    Zhenghe Li

    Article

    Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

    Insert

    tobacco etch virus P1/HC-Pro
    

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV pEF1a-DIO-FLPo-WPRE-hGHpA

    Plasmid

    #87306

    Purpose

    An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

    Depositor

    Li Zhang

    Article

    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre-dependent mouse codon-optimized Flp recombinase.
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX

    Plasmid

    #164611

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Expression

    Mammalian

    Mutation

    S383T

    Promoter

    CMV

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Pepper-fused sgRNA

    Plasmid

    #217520

    Purpose

    Expresses Pepper-fused sgRNA in mammalian cells with U6 promoter

    Depositor

    Xing Li

    Article

    Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

    Insert

    Pepper-fused sgRNA for spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. plenti-ef1a-dCasRx-FTO-HA-T2A-BSD

    Plasmid

    #177120

    Purpose

    dCasRx mediated RNA tethering of FTO

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    dCasRx-FTO
    

    Use

    Lentiviral

    Tags

    HA and SV40 NLS

    Mutation

    dCasRX contains R239A/H244A/R858A/H863A mutations…

    Promoter

    Ef1a

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. miniCMV-tdTomato-tDeg

    Plasmid

    #217521

    Purpose

    Expresses tdTomato-tDeg fluorogenic protein in mammalian cells with miniCMV promoter

    Depositor

    Xing Li

    Article

    Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

    Insert

    tdTomato-tDeg fluorogenic protein
    

    Expression

    Mammalian

    Promoter

    miniCMV

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-hSyn-GRAB-rDA3h

    Plasmid

    #208703

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3h
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pL

    Plasmid

    #196286

    Purpose

    35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV L antigenome encoding codon-optimized L (RdRp)
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQhsCDtRNA-

    Plasmid

    #86015

    Purpose

    Multi-gene expression system with human box C/D proteins with U14

    Depositor

    Hong Li

    Article

    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:

    Inserts

    U14 (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    

    Use

    Co-expression; purification

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    amino acids 1-401, amino acids 1-411, and amino a…

    Available Since

    Jan. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDisplay-EGFP-CAAX

    Plasmid

    #208683

    Purpose

    Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    membrane-localized enhanced green fluorescent protein EGFP-CAAX
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDisplay-GRAB_CRFmut

    Plasmid

    #208656

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge