Addgene

We narrowed to 173 results for: MET1

Showing: 41 - 60 of 173 results

Results per page:

Sort results by:

ClhN-P1K-3VSV-Atg8(R65A P85L P86L)-MET15

Plasmid

#207053

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Use

Tags

3xVSV

Expression

Yeast

Mutation

R65A P85L P86L

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-P1K-3VSV-Atg8(L55W T56A V61W R65A)-MET15

Plasmid

#207054

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Use

Tags

3xVSV

Expression

Yeast

Mutation

L55W T56A V61W R65A

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINB-OTULIN (full-length, aa 1-352)

Plasmid

#61464

Purpose

Expresses human OTULIN (full-length) in E. coli.

Depositor

David Komander

Article

Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

Insert

OTULIN (OTULIN Human)

Use

Tags

His6-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK367

Plasmid

#72406

Purpose

Produces Acetobacter aceti 1023 Est06

Depositor

T. J. Kappock

Insert

lipase

Use

Tags

Expression

Bacterial

Mutation

lacks Met1 and Thr2 in annotated (v1) sequence

Promoter

T7

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK407

Plasmid

#72421

Purpose

Produces Acetobacter aceti 1023 Est06, C-terminal His6 tag

Depositor

T. J. Kappock

Insert

lipase

Use

Tags

His6

Expression

Bacterial

Mutation

lacks Met1 and Thr2 in annotated (v1) sequence

Promoter

T7

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK439

Plasmid

#72478

Purpose

Produces Thermoplasma acidophilum citrate synthase pseudo-dimer

Depositor

T. J. Kappock

Insert

citrate synthase (artificial dimer)

Use

Tags

Expression

Bacterial

Mutation

inserted NSTCLVPRGSPV sequence between C- and N-t…

Promoter

T7

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1_4x7SKPylT_EF1_Ub-*NoBody

Plasmid

#162807

Purpose

STELLA plasmid for amber suppression of N-terminally labelled NoBody

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)NoBody

Use

Piggybac

Tags

Expression

Mammalian

Mutation

NoBody (Met1, Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1_4x7SKPylT_EF1_Ub-*GFP

Plasmid

#162806

Purpose

STELLA plasmid for amber suppression of N-terminally labelled GFP

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

EF1_Ub-(N-TAG)GFP

Use

Piggybac

Tags

Expression

Mammalian

Mutation

GFP (Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
LifeAct-mCherry

Plasmid

#193723

Purpose

Labeling microtubules in living mammalian cells

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

LifeAct-mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

mCherry: Met1 Deleted

Promoter

Chicken beta-actin

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1_4x7SKPylT_EF1_Ub-*SMIM38

Plasmid

#162808

Purpose

STELLA plasmid for amber suppression of N-terminally labelled SMIM38

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)SMIM38

Use

Piggybac

Tags

Expression

Mammalian

Mutation

SMIM38 (Met1, Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS_4x7SKPylT_EF1_Ub-*PIGBOS

Plasmid

#162809

Purpose

STELLA plasmid for amber suppression of N-terminally labelled PIGBOS

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)PIGBOS

Use

Piggybac

Tags

Expression

Mammalian

Mutation

PIGBOS (Met1, Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pB80-2xFKBP-L-mCherry-CAAX

Plasmid

#193713

Purpose

Rapalog-inducible coupling to plasma membrane (CAAX) via FKBP

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

2xFKBP-L-mCherry-CAAX

Use

Tags

2xFKBP-mCherry

Expression

Mammalian

Mutation

mCherry: Met1 Deleted

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1_4x7SKPylT_EF1_Ub-*CoV2_M

Plasmid

#162815

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_M

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_M

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_M(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b

Plasmid

#162816

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_9b

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_9b

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_9b(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6

Plasmid

#162817

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF6

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF6(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b

Plasmid

#162818

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7b

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF7b

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF7b(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10

Plasmid

#162819

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF10

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF10(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt

Plasmid

#162821

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_alt

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF3b_alt

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF3b_alt(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
KHBD00646

Plasmid

#39708

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1705 (Met Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14

Plasmid

#162820

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF9c/14

Use

Piggybac

Tags

Expression

Mammalian

Mutation

CoV2_ORF9c/14(Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge