Addgene

We narrowed to 1,025 results for: PRK5

Showing: 41 - 60 of 1025 results

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pRK5-mWnt1

Plasmid

#42273

Depositor

Jeremy Nathans, Chris Garcia

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt1 (Wnt1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-mWnt8b

Plasmid

#42285

Depositor

Jeremy Nathans, Chris Garcia

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt8b (Wnt8b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt10b

Plasmid

#42289

Depositor

Jeremy Nathans, Chris Garcia

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt10b (Wnt10b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-mWnt8a

Plasmid

#42284

Depositor

Jeremy Nathans, Chris Garcia

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt8a (Wnt8a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
PRK5 I7_pECIA2

Plasmid

#114982

Purpose

Bait vector PRK5 I7_pECIA2 should be used with prey vector PRK5 I7_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G50610

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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PRK5 I7_pECIA14

Plasmid

#114782

Purpose

Prey vector PRK5 I7_pECIA14 should be used with bait vector PRK5 I7_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G50610

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARβ

Plasmid

#119914

Purpose

Mammalian expression of the tAKARβ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARβ

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-3(β2)

Plasmid

#160268

Purpose

Expresses a concatemeric construct of a α4:β2 (2:3) nAChR

Depositor

Pierre-Jean Corringer

Article

Prevost et al Cell Mol Life Sci. 2020 May 29. pii: 10.

Insert

3(β2) concatemer

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-3(α4)

Plasmid

#160267

Purpose

Expresses a concatemeric construct of a α4:β2 (3:2) nAChR

Depositor

Pierre-Jean Corringer

Article

Prevost et al Cell Mol Life Sci. 2020 May 29. pii: 10.

Insert

3(α4) concatemer

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKAR4α

Plasmid

#119919

Purpose

Mammalian expression of the tAKAR4α sensor for detecting PKA activity using ratiometric imaging

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKAR4α

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARα

Plasmid

#119913

Purpose

Mammalian expression of the tAKARα sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARα

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARγ

Plasmid

#119915

Purpose

Mammalian expression of the tAKARγ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARγ

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARζ

Plasmid

#119918

Purpose

Mammalian expression of the tAKARζ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARζ

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARδ

Plasmid

#119916

Purpose

Mammalian expression of the tAKARδ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARδ

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARε

Plasmid

#119917

Purpose

Mammalian expression of the tAKARε sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARε

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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HA-GLUT5 in pRK5

Plasmid

#154205

Purpose

N-terminal HA-tagged GLUT5 cDNA expression vector for transfections

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

SLC2A5 (SLC2A5 Human)

Use

Tags

N terminal HA tag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-Ufm1 ∆C3

Plasmid

#133866

Purpose

Expression of human UFM1 mutant lacking the last three C-terminal residues

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

Ubiquitin-fold modifier 1 lacking last three C-terminal residues (UFM1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-metap2

Plasmid

#100512

Purpose

Expresses HA tagged metap2 in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

Metap2 (METAP2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-WT

Plasmid

#17608

Purpose

Mammalian expression of HA tagged ubiquitin

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-KO

Plasmid

#17603

Purpose

Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

KO: all lysines mutated to arginines

Promoter

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge