We narrowed to 273 results for: T7 rna polymerase
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Plasmid#101679PurposeLAA-tagged mCherry under T7 promoter controlDepositorInsertLAA-tagged mCherry under T7 promoter control
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE-WT-CGG-R12-KIR
Plasmid#63626PurposeCGG-R12-KIR RNAP mutantDepositorInsertK1F IKR RNAP
MutationQ744K, L747V, N748H, L749I, R756E, L757M, H772RPromoterT5/LacAvailable SinceJuly 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE-WT-CGG-R12-KIRV
Plasmid#63668PurposeCGG-R12-KIRV RNAP mutantDepositorInsertCGG-R12-KIRV RNAP
MutationQ744K, L747V, N748H, L749I, R756E, L757M, H772R, …PromoterT5/LacAvailable SinceApril 15, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE-WT-T3-R5-RV
Plasmid#63628PurposeT3-R5-RV RNAP mutantDepositorInsertT3-R5-RV RNAP
MutationT745K, N748D, L749M, M750I, H772R, E775VPromoterT5/LacAvailable SinceApril 15, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE-WT-T3-R5-RRVH
Plasmid#63623PurposeT3-R5-RRVH RNAP mutantDepositorInsertT3 RRVH RNAP
MutationT745K, N748D, L749M, M750I, G753R, H772R, E775V, …PromoterT5/LacAvailable SinceJuly 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE-WT-KIF-R5-IRH
Plasmid#63629PurposeK1F-R5-IRH RNAP mutantDepositorInsertKIF-R5-IRH RNAP
MutationL749I, Q754S, R756N, I761V, H772R, and Q786HPromoterT5/LacAvailable SinceJune 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE-WT-KIF-R5-KIKR
Plasmid#63624PurposeKIF-R5-KIKR RNAP mutantDepositorInsertKIF-R5-KIKR RNAP
MutationQ744K, L749I, M750K, Q754S, R756N, I761V, H772RPromoterT5/LacAvailable SinceApril 15, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTHSSd_23
Plasmid#59954PurposePTac T7 sigma fragment expressionDepositorInsertT7 sigma fragment
ExpressionBacterialMutationN/APromoterPTacAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
MC177VSG221RNAi
Plasmid#59730PurposeFor use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.DepositorInsertVSG221 RNAi fragment
UseRNAiPromoteropposing T7 promotersAvailable SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE-WT-CTGA-R13-AKSIRV
Plasmid#63627PurposeCTGA-R13-AKSIRV RNAP mutantDepositorInsertCTGA-R13-AKSIRV RNAP
Tagsn/aMutationV725A, Q744K, L747I, N748S, L749I, R756T, Q758K, …PromoterT5/LacAvailable SinceJune 3, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE-WT-N4-R5-RNRV
Plasmid#63630PurposeN4-R5-RNRV RNAP mutantDepositorInsertN4-R5-RNRV RNAP
MutationL747I, N748D, L749C, M750V, F751I, Q754T, F755R, …PromoterT5/LacAvailable SinceApril 15, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE-WT-N4-R5-YRNRV
Plasmid#63625PurposeN4-R5-YRNRV RNAP mutantDepositorInsertN4-R5-YRNRV RNAP
MutationN671Y, L747I, N748D, L749C, M750V, F751I, Q754T, …PromoterT5/LacAvailable SinceJune 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE-WT-CTGA-R13-AKSRV
Plasmid#63622PurposeCTGA-R13-AKSRV RNAP mutantDepositorInsertCTGA-R13-AKSRV RNAP
ExpressionBacterialMutationV725A, Q744K, L747I, N748S, R756T, Q758K, H772R, …PromoterT5/LacAvailable SinceJuly 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTHSSd_44
Plasmid#59966Purposeconstitutive expression of T7 sigma fragmentDepositorInsertT7 sigma fragment
ExpressionBacterialMutationN/APromoterPcon sigmaAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZB641_pT7_Lysin-KU1
Plasmid#228511PurposeT7 RNAP inducible Lysin KU1 ExpressionDepositorInsertLysin KU1
UseSynthetic BiologyPromoterT7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
p6-8
Plasmid#118089Purposerapa-T7- mRNA(GFP)DepositorInsertRapamycin-inducible split T7 RNAP with T7 driven mRNA for GFP
ExpressionMammalianAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTSara-C
Plasmid#60722PurposeExpresses the C-terminal fragment of split T7 RNAP split a position 179. Insert is driven by the arabinose inducible promoter PBAD. Contains a constitutive araC ORFDepositorInsertResidues 180-880 of T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationOnly amino acids 180-880 of T7 RNAPPromoterPBADAvailable SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSara-N
Plasmid#60721PurposeExpresses the N-terminal fragment of split T7 RNAP split a position 179. Insert is driven by PBAD. Contains a constitutive araC ORFDepositorInsertResidues 1-179 of T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationOnly amino acids 1-179 of T7 RNAPPromoterPBADAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-wt
Plasmid#60728PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…Available SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
BioBrick pET-24a(+)
Plasmid#117802PurposeBioBrick pET vector for protein overexpression in E. coliDepositorTypeEmpty backboneUseEscherichia coliAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only