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Plasmid#209701PurposeExpresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent mannerDepositorInserthM4D(Gi)
UseAAVTagsmCherryExpressionMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBA-DIO-hM4Di-mCherry
Plasmid#81008PurposeCre-dependent expression of hM4Di-mCherry under CBA promoterDepositorInserthM4D(Gi)-mCherry
UseAAVTagsmCherryExpressionMutationPromoterCBAAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D
Plasmid#52521PurposeCo-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virusDepositorInsertChR2-2a-hM4D
UseAAV and Cre/LoxTags2xHA on ChR2ExpressionMutationPromoterCBAAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi1-91-NLuc
Plasmid#207148PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAI1 (GNAI1 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)KPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBScMXT + mSlo1 MBr5/3
Plasmid#16149DepositorInsertMus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse brain)
UseXenopus oocyte expressionTagsExpressionMutationN/APromoterAvailable sinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-3-GST-DmGW182_1-593_F
Plasmid#146271PurposeBacterial Expression of DmGW182_1-593DepositorInsertDmGW182_1-593 (gw Fly)
UseTagsExpressionBacterialMutationone non silent mutation T1772A (L591Q) compared t…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi1-LgB91
Plasmid#134342PurposeNanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.DepositorInsertGαi1-LgB91 (GNAI1 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot2_1-401_I
Plasmid#146616PurposeInsect Expression of DmNot2_1-401DepositorInsertDmNot2_1-401 (Rga Fly)
UseTagsExpressionInsectMutationone silent mutation C267T compared to the ORF ext…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_MYL6_EF-hand
Plasmid#109794PurposeProtein expression and purification of MYL6_EF-handDepositorInsertMYL6_EF-hand (MYL6 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-TRPV1-P2A-DsRed
Plasmid#200830PurposeTransduce all neuron types with AAV to express TRPV1 along with a DsRed fluorescence reporterDepositorInsertTRPV1-P2A-DsRed (Trpv1 Rat)
UseAAVTagsExpressionMammalianMutationPromoterSynAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAI1_G-alpha
Plasmid#110012PurposeProtein expression and purification of GNAI1_G-alphaDepositorInsertGNAI1_G-alpha (GNAI1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-DIO-TRPV1-P2A-mCherry
Plasmid#200831PurposeIn the presence of Cre, drives neuron-specific expression of TRPV1 with fluorescence reporter mCherryDepositorInsertTRPV1-P2A-mCherry (Trpv1 Rat)
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
p-OiRS3GG
Plasmid#98589PurposeExpresses default target RNA (gI intron) and pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).DepositorInsertsgroup I intron
PheS cassette to be selectively replaced by unique asRNA probes
UseTagsExpressionBacterialMutationPromoterpBAD and pLtetOAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mEYFP(Q69K)
Plasmid#190755PurposeExpression of trimeric G protein with mCherry2 (GNB1 and GNG2) or mEYFP(Q69K) (GNAI1) tags.DepositorUseTagsmCherry2 and mEYFP(Q69K)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphai1-RLuc8
Plasmid#140973PurposeEncodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphai1-RLuc8 (GNAI1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-6xTRE-FLEX-hM4Di.mCherry
Plasmid#169415PurposeActivity-dependent, cre-dependent, temporally restricted inhibitory DREADD expressionDepositorInserthM4Di.mCherry
UseAAVTagsmCherryExpressionMutationhM4Di is Gi-coupled DREADD.Derived from human mus…PromoterMinimal TATAAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AF10_OM-LZ
Plasmid#111753PurposeBacterial expression for structure determinationDepositorInsertMLLT10 (MLLT10 Human)
UseTags6x His - TEVExpressionBacterialMutationContains the OM-LZ region of AF10 (residues 720-7…PromoterT7Available sinceJune 14, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
Gi1-CASE
Plasmid#168120PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A121/E122 within GNAI1PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gi3-CASE
Plasmid#168122PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A114/E115 within GNAI1PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gai1
Plasmid#196048PurposeEncodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only