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Showing: 1 - 20 of 23 results

  1. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...single gene knockout. Top AAV: pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost... of these viruses are popular, pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double floxed human muscarinic... promoter. Purpose of this AAV pAAV-hSyn-DIO-hM4D(Gi)-mCherry can be used to induce neuronal silencing...types (Aschauer et al., 2013). Expression of hM4D(Gi)-mCherry from this virus is cre-dependent, meaning...al., 2011). Viruses expressing pAAV-hSyn-DIO-hM4D(Gi)-mCherry are typically injected into brain regions...intravenously. Tips for using pAAV-hSyn-DIO-hM4D(Gi)-mCherry When using this virus for the first time...endogenous mCherry fluorescence when visualizing hM4D(Gi)-mCherry in fixed tissue. References 1. Aschauer...

  2. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...Fishman GI, Conklin BR (1999) Conditional expression and signaling of a specifically designed Gi-coupled... Ross, S. E., Davis, B. M., & Gold, M. S. (2016). Gi-DREADD Expression in Peripheral Nerves Produces Ligand-Dependent...A (2019) Olanzapine: A potent agonist at the hM4D(Gi) DREADD amenable to clinical Additional resources...

  3. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...75033  AAV1 pAAV CD68-hM4D(Gi)-mCherry 50467  AAV2 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine 121538  AAV5... AAV5 pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) 121539  AAV5 pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) New chemogenetics...

  4. Chemogenetics AAV Preps

    Type
    Collection
    ...pAAV-GFAP-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion none 5 Roth 177672 pAAV-nEF-Con/Fon DREADD Gi-mCherry...rg*, PHPeB Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion Cre-dependent...Cre-dependent 8 Roth 50455 pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine..., 5, 8, 9, rg* Roth 50475 pAAV-hSyn-hM4D(Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion none 1, 2, 5...-2a-GFP hM4D(Gi) - Inhibition GFP Cre-dependent 1 Sternson 154867 pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA...EGFP none 5 Sternson 121538 pAAV SYN1 HA-hM4D(Gi) hM4D(Gi) - Inhibition HA fusion none 2, 5 Richie 121539..., 5 Richie 50467 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine...

  5. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...Chemogenetics Bryan Roth New serotype pAAV-CaMKIIa-hM4D(Gi)-mCherry AAV1 Chemogenetics Bryan Roth New serotype...Chemogenetics Bryan Roth New serotype pAAV-hSyn-hM4D(Gi)-mCherry AAV1 Chemogenetics Bryan Roth New serotype...

  6. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...Plasmid Serotype Name 75033  AAV1  pAAV CD68-hM4D(Gi)-mCherry (CD68 is a  microglia/macrophage promoter...Gq) 121538  AAV5  pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) 119741  AAV5  AAV SYN flex PSAM4 GlyR IRES EGFP...

  7. Chemogenetics Plasmids

    Type
    Collection
    ...collection: hM3D(Gq), rM3D(Gs), hM4D(Gi) and KORD. hM3D(Gq), rM3D(Gs), and hM4D(Gi) are all based on muscarinic...and couples to the Gi G-protein. hM3D(Gq) and rM3D(Gs) are activating, while hM4D(Gi) and KORD are inhibitory...muscarinic receptors and couple to the Gi, Gq, or Gs G-proteins, respectively. KORD is derived from the κ-opoid...

  9. New Viral Vectors - March 2024

    Type
    Blog Post
    ...Adesnik New viral vector pAAV-nEF-Con/Foff DREADD Gi-mCherry AAV8 Chemogenetics Deisseroth New viral vector...

  11. Brain Initiative Collection

    Type
    Collection
    ...Bryan Roth 75033-AAV1 pAAV CD68-hM4D(Gi)-mCherry Expression of hM4D(Gi) using the CD68 promoter. Bryan Roth...Roth 75033-AAV9 pAAV CD68-hM4D(Gi)-mCherry Expression of hM4D(Gi) using the CD68 promoter. Bryan Roth ...Fishell 83896-AAV1 pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain...Fishell 83896-AAV9 pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain... 83896-AAVrg pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain...

  13. Hot Plasmids - October 2022

    Type
    Blog Post
    ...Addgene.   Find anti-c-Myc antibody here!   Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal...

  14. Retrograde AAV viral preps

    Type
    Collection
    ...50475 pAAV-hSyn-hM4D(Gi)-mCherry Syn Inhibitor DREADD Roth 154867 pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA Syn...Cre-dependent DREADD Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor, Cre-dependent DREADD Roth...

  15. Deisseroth INTRSECT Collection

    Type
    Collection
    ...-nEF-Coff/Fon DREADD Gi-mCherry Flp AND NOT Cre 177672 pAAV-nEF-Con/Fon DREADD Gi-mCherry Cre AND Flp ...Flp 177673 pAAV-nEF-Con/Foff DREADD Gi-mCherry Cre AND NOT Flp 183532 pAAV-nEF Con/Fon DREADD Gq-mCherry...

  19. Of Myc and Men

    Type
    Blog Post
    ...key.    References and Resources References Evan GI, Lewis GK, Ramsay G, Bishop JM (1985) Isolation of...

  20. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...targets members of all four G protein families: Gs, Gi/o, Gq/11, and G12/13. Find the ONE-GO Biosensors ...
Showing: 1 - 20 of 23 results