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Showing: 1 - 15 of 15 results

  1. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...single gene knockout. Top AAV: pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost... of these viruses are popular, pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double floxed human muscarinic... promoter. Purpose of this AAV pAAV-hSyn-DIO-hM4D(Gi)-mCherry can be used to induce neuronal silencing...types (Aschauer et al., 2013). Expression of hM4D(Gi)-mCherry from this virus is cre-dependent, meaning...al., 2011). Viruses expressing pAAV-hSyn-DIO-hM4D(Gi)-mCherry are typically injected into brain regions...intravenously. Tips for using pAAV-hSyn-DIO-hM4D(Gi)-mCherry When using this virus for the first time...endogenous mCherry fluorescence when visualizing hM4D(Gi)-mCherry in fixed tissue. References 1. Aschauer...

  2. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...Fishman GI, Conklin BR (1999) Conditional expression and signaling of a specifically designed Gi-coupled... Ross, S. E., Davis, B. M., & Gold, M. S. (2016). Gi-DREADD Expression in Peripheral Nerves Produces Ligand-Dependent...A (2019) Olanzapine: A potent agonist at the hM4D(Gi) DREADD amenable to clinical Additional resources...

  3. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...75033  AAV1 pAAV CD68-hM4D(Gi)-mCherry 50467  AAV2 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine 121538  AAV5... AAV5 pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) 121539  AAV5 pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) New chemogenetics...

  4. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...Plasmid Serotype Name 75033  AAV1  pAAV CD68-hM4D(Gi)-mCherry (CD68 is a  microglia/macrophage promoter...Gq) 121538  AAV5  pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) 119741  AAV5  AAV SYN flex PSAM4 GlyR IRES EGFP...

  5. New Viral Vectors - Winter 2025

    Type
    Blog Post
    Published
    Jan. 28, 2025, noon
    ...Chemogenetics Bryan Roth New serotype pAAV-CaMKIIa-hM4D(Gi)-mCherry AAV1 Chemogenetics Bryan Roth New serotype...Chemogenetics Bryan Roth New serotype pAAV-hSyn-hM4D(Gi)-mCherry AAV1 Chemogenetics Bryan Roth New serotype...

  7. New Viral Vectors - March 2024

    Type
    Blog Post
    Published
    March 5, 2024, 2:15 p.m.
    ...Adesnik New viral vector pAAV-nEF-Con/Foff DREADD Gi-mCherry AAV8 Chemogenetics Deisseroth New viral vector...

  8. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...shown incredible growth this year. pAAV-hSyn-DIO-hM4D(Gi)-mCherry was the top requested AAV prep for the 2nd...

  10. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...Addgene.   Find anti-c-Myc antibody here!   Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal...

  11. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...Plasmid Serotype Name 44362 AAV1 pAAV-hSyn-DIO-hM4D(Gi)-mCherry 83896 AAVrg pAAV-hDlx-GiDREADD-dTomato-Fishell...

  14. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...key.    References and Resources References Evan GI, Lewis GK, Ramsay G, Bishop JM (1985) Isolation of...

  15. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...targets members of all four G protein families: Gs, Gi/o, Gq/11, and G12/13. Find the ONE-GO Biosensors ...
Showing: 1 - 15 of 15 results