Addgene

We narrowed to 1,127 results for: cas12

Showing: 581 - 600 of 1127 results

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pLCHKO_TK1_HPRT1_Lb_2

Plasmid

#155060

Purpose

Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and an intergenic site & HPRT1 using LbCas12a (CHyMErA system)

Depositor

Jason Moffat, Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ132D-RZ-Lb

Plasmid

#134348

Purpose

Golden Gate entry vector; empty vector to clone the 2nd LbCas12a crRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Li et al Plant Biotechnol J. 2019 Nov 15. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ131C-RZ-Lb

Plasmid

#134347

Purpose

Golden Gate entry vector; empty vector to clone the 1st LbCas12a crRNA under OsU6 promoter

Depositor

Yiping Qi

Article

Li et al Plant Biotechnol J. 2019 Nov 15. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJC005.em

Plasmid

#182738

Purpose

CRISPR-Cas12a genome editing of E. faecium

Depositor

James Collins

Article

Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pJC005.gent

Plasmid

#182739

Purpose

CRISPR-Cas12a genome editing of E. faecium

Depositor

James Collins

Article

Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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EC67841

Plasmid

#209512

Purpose

Cloning in complete guide expression cassettes, with Cas12a (D156R)

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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EC67842

Plasmid

#209516

Purpose

Cloning in complete guide expression cassettes, with Cas12a (D156R)

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCFD8

Plasmid

#140619

Purpose

Expression of one of several LbCas12a crRNAs in Drosophila

Depositor

Michael Boutros

Article

Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Drosophila U6:3

Available since

Oct. 10, 2020

Availability

Academic Institutions and Nonprofits only

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EC67843

Plasmid

#209513

Purpose

Cloning in complete guide expression cassettes, with Cas12a (D156R)

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC64434

Plasmid

#209518

Purpose

Cloning in complete guide expression cassettes, with Cas9 & Cas12a(D156R)

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDF850

Plasmid

#228713

Purpose

Mammalian expression of EVOLVEpro PsaCas12f (epPsaCas12f)

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

epPsaCas12f

Use

CRISPR

Tags

Expression

Mammalian

Mutation

I178A/K333V/K454P

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSL3287

Plasmid

#139750

Purpose

Base Cas12a CRISPRi plasmid

Depositor

Himadri Pakrasi

Article

Knoot et al ACS Synth Biol. 2020 Jan 17;9(1):132-143

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSEVA451-Cpf1

Plasmid

#192362

Purpose

CRISPR-based vector for genome editing

Depositor

Juana María Navarro Llorens

Article

Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

Insert

Cas12a, gRNA scaffold

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVA351-Cpf1

Plasmid

#192361

Purpose

CRISPR-based vector for genome editing

Depositor

Juana María Navarro Llorens

Article

Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

Insert

Cas12a, gRNA scaffold

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pPfetFn-Target_v2

Plasmid

#199564

Purpose

gRNA expression for FnCas12a in E. coli and clostridia

Depositor

Philippe Lambin

Article

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

SacB

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pthl_fU

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTarget12f1

Plasmid

#171183

Purpose

Constitutive expression of sgRNA without donor DNA

Depositor

Kohsuke Honda

Article

Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

Insert

sgRNA scaffold in the CRISPR/Cas12f1 system

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119(SpeI) promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pGEL636

Plasmid

#189830

Purpose

The T-DNA backbone of Cas12j2 for transcriptional activation in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

pZmUbi1

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AksgRNA

Plasmid

#121955

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b single chimeric gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AasgRNA

Plasmid

#121954

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AasgRNA3.8

Plasmid

#121958

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA, short version

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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