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Plasmid#92305PurposeExpression of humanized Mb3Cpf1 and crRNADepositorInserthuMb3Cpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY127 (pcDNA3.1-U6-huTsCpf1)
Plasmid#92303PurposeExpression of humanized TsCpf1 and crRNADepositorInserthuTsCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_SSRP1
Plasmid#72370PurposeDonor vector for 3' FLAG tag of human SSRP1DepositorInsertSSRP1 Homology Arms (SSRP1 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
GuideVector_BbsI
Plasmid#110692PurposeFor rapid cloning and expression of sgRNAs in mammalian cells. Contains copGFP for easy FACS sorting.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-EFS-SpCas9-P2A-blast
Plasmid#106477Purposesingle-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screensDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZNF219
Plasmid#72367PurposeDonor vector for 3' FLAG tag of human ZNF219DepositorInsertZNF219 Homology Arms (ZNF219 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_TGIF2
Plasmid#72373PurposeDonor vector for 3' FLAG tag of human TGIF2DepositorInsertTGIF2 Homology Arms (TGIF2 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZNF146
Plasmid#72359PurposeDonor vector for 3' FLAG tag of human ZNF146DepositorInsertZNF146 Homology Arms (ZNF146 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTD56
Plasmid#172895PurposemKate2^SEC^AID* vector with ccdB markers for cloning homology armsDepositorInsertmKate2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTD72
Plasmid#172896PurposemKate2^SEC^2xHA vector with ccdB markers for cloning homology armsDepositorInsertmKate2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_PBX2
Plasmid#72356PurposeDonor vector for 3' FLAG tag of human PBX2DepositorInsertPBX2 (PBX2 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJAT39
Plasmid#204289PurposeFor marking CRISPR HDR integrant with EGFP expressed in flight muscles.DepositorInsertMHC-EGFP+H3:H26
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT10
Plasmid#204293PurposeFor marking CRISPR HDR integrant with DsRed expressed in flight muscles.DepositorInsertMHC-DsRed
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa VP64 Afp
Plasmid#99697PurposeExpresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAVDepositorInsertdCas9 and gRNA targeting Afp (Afp Synthetic)
UseAAV and CRISPRTagsVP64ExpressionMutationdead Cas9PromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pnCas9-AID
Plasmid#196242PurposePlasmid expressing the nCas9-AID fusionDepositorInsertnickase Cas9 fused to AID
UseCRISPRTagsExpressionBacterialMutationPromotertac promoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR_AtU6p_BsaI_gRNA
Plasmid#225987PurposeTo make Gateway entry clone of CRISPR guide RNA under AtU6pDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1
Plasmid#184294PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1DepositorInsertsgRNA-Adrb1
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Grp
Plasmid#184292PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting GrpDepositorInsertsgRNA-Grp
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Oprl1
Plasmid#184291PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Oprl1DepositorInsertsgRNA-Oprl1
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Rxfp3
Plasmid#184290PurposeA single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Rxfp3DepositorInsertsgRNA-Rxfp3
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only