We narrowed to 7,283 results for: ef1a

1. pLV-EF1a-H2B-miRFP670

   Plasmid

   #212668

   Purpose

   Express tagged H2B

   Depositor

   Tobias Meyer, Yumi Konagaya

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   H2B (H2BC11 Human)
   

   Use

   Lentiviral

   Tags

   miRFP670

   Expression

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode

   Plasmid

   #170237

   Purpose

   Barcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   KRAS (KRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   G12V

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRE-STOP EF1a-rtTA3

   Plasmid

   #195882

   Purpose

   control construct of TRE-VEGFA EF1a-rtTA3

   Depositor

   Elaine Fuchs

   Article

   Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

   Insert

   VEGFA (Vegfa Mouse, Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a-ABCD3-RFP-Puro

   Plasmid

   #170168

   Purpose

   Mammalian Expression of Ef1a-ABCD3-RFP-Puro

   Depositor

   Christopher Moxham

   Insert

   ABCD3-RFP-Puro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-DIO-mGreenLantern

   Plasmid

   #164468

   Purpose

   Cre-dependent EF1a-driven expression of mGreenLantern in animals using AAV

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   EF1a

   Available since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO_EGFP-GFE3

   Plasmid

   #79871

   Purpose

   For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-GFE3
   

   Use

   AAV, Cre/Lox, and Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. LV-Sox10MCS5-GFP-EF1a-mCherry

   Plasmid

   #115782

   Purpose

   Sox10-MCS5-GFP and a constitutive EF1α-mCherry reporter plasmid. Construct generates constitutive red fluorescence and basal promoter cfos conjugated SOXMCS5 enhancer driven GFP expression in a cell.

   Depositor

   Fraser Sim

   Article

   Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1

   Inserts

   mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
   EF1alpha promoter driven constitutive mCherry
   

   Use

   Lentiviral

   Tags

   EF1α promoter fused to mCherry and cfos promoter …

   Expression

   Mutation

   Promoter

   SOX10 Multiple Species Conserved enhancer element…

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAIDFA-EF1a-NmScarlet-mAID

   Plasmid

   #140615

   Purpose

   Expression of OsTIR1F74A and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   OsTIR1 mutated F74A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

   Plasmid

   #217635

   Purpose

   AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

   Depositor

   Martin Kampmann

   Insert

   mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1alpha and mU6

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-DIO-mCherry

    Plasmid

    #50462

    Purpose

    Double floxed mCherry under the control of EF1a promoter

    Depositor

    Bryan Roth

    Insert

    mCherry
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB-EF1a-NLuc-IRES-Puro

    Plasmid

    #130936

    Purpose

    PiggyBac vector for constitutive NanoLuc expression in mammalian cells

    Depositor

    James Thomson

    Article

    Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

    Insert

    NanoLuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-fDIO EYFP

    Plasmid

    #55641

    Purpose

    Flp-Dependent EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEnt L1L3 EF1a-tTA-2

    Plasmid

    #26803

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    Elongation Factor 1 alpha promoter and tTA transgene
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSMPUW_6.17_Ex_Ef1a-v5-EGFP-SV40-Zeo

    Plasmid

    #128453

    Purpose

    lentiviral expression vector with Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and ZeoResistance gene

    Depositor

    Lindy Barrett

    Insert

    Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-EF1a-eFFly-eGFP

    Plasmid

    #104834

    Purpose

    eFFly luciferase for invivo Imaging, eGFP for flourescent detection

    Depositor

    Irmela Jeremias

    Article

    Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

    Inserts

    eFFly
    eGFP
    

    Use

    Lentiviral and Luciferase; Egfp

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-fDIO-Cre

    Plasmid

    #121675

    Purpose

    Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

    Depositor

    Alexander Nectow, Esteban Engel

    Article

    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

    Has Service

    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

    Insert

    NLS-CRE-HA
    

    Use

    AAV

    Tags

    3xHA and SV40-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-EF1a-hMLH1dn-Hygro

    Plasmid

    #195512

    Purpose

    Lentiviral expression plasmid of hMLH1dn with P2A-HygroR marker

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    hMLH1dn-P2A-HygroR (MLH1 Human)
    

    Use

    Lentiviral

    Tags

    P2A

    Expression

    Mammalian

    Mutation

    Human MLH1 del754-756

    Promoter

    EF-1a

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-EF1a-jGCaMP8m-WPRE

    Plasmid

    #176757

    Purpose

    AAV-mediated expression of GCaMP8m under the EF1a promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-EF1a-hMLH1dn-eGFP

    Plasmid

    #191104

    Purpose

    Lentiviral expression plasmid of hMLH1dn with P2A-eGFP reporter

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    hMLH1dn
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode

    Plasmid

    #170248

    Purpose

    Barcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    C124S

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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