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pLenti-dCas9-T2A-GFP
Plasmid#193781PurposeTricistronic expression of dCas9-EGFP-BSDDepositorInsertdead Cas9
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterIn frame with existing Cas9 promoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin
Plasmid#211471Purposelentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistanceDepositorInsertCas9_T2A_mNeonGreen_P2A_BlasticidinR
UseCRISPR and LentiviralTags3xFlagExpressionMammalianMutationPromoterhU6, EF-1aAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-T2A-mApple
Plasmid#193780PurposeTricistronic expression of Cas9-mApple-BSD in mammalian cellsDepositorInsertmApple
UseLentiviralTagsExpressionMammalianMutationPromoterIn frame with Cas9Available sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUDE710
Plasmid#103020Purposeexpression of a Cpf1 programming crRNA targeting ADE2 and HIS4 (crADE2-3 crHIS4-4.S)DepositorUseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-puro_Panc480-MT7
Plasmid#200941PurposeMultiplexed CRISPR array expressing 7 sgRNA in a lentiviral backboneDepositorInsertmultiplexed sgRNA array
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6, EF-1aAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
U17m
Plasmid#121023PurposeMoClo golden gate assembly BC part for Bujard RBS (strong RBS from https://doi.org/10.1093/nar/25.6.1203). Please see Supplemental Documents for annotated Genbank file.DepositorInsertBujard RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
MG1655-tMMR
Bacterial Strain#62392PurposeE. coli MG1655 temperature-sensitive mismatch repair deficient strain, harboring mutS(A134V) mutL(G62S), and nadA::Tn10 pglΔ8 [λ cI857 Δ(cro-bioA)]} for λ Red expressionDepositorBacterial ResistanceTetracyclineSpeciesEscherichia coliAvailable sinceMarch 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMS26
Plasmid#32295DepositorTypeEmpty backboneUseBacterial gene additionTagsExpressionBacterialMutationPromoterrhaBAvailable sinceSept. 26, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
HL 1745
Bacterial Strain#61160PurposeLim Lab StrainDepositorBacterial ResistanceNoneAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
HL 1852
Bacterial Strain#61161PurposeLim Lab StrainDepositorBacterial ResistanceNoneAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
HL 1951
Bacterial Strain#61162PurposeLim Lab StrainDepositorBacterial ResistanceNoneAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
HL 2028
Bacterial Strain#61163PurposeLim Lab StrainDepositorBacterial ResistanceNoneAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
HL 5220
Bacterial Strain#61164PurposeLim Lab StrainDepositorBacterial ResistanceNoneAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
HL 5221
Bacterial Strain#61165PurposeLim Lab StrainDepositorBacterial ResistanceNoneAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
HL 5222
Bacterial Strain#61166PurposeLim Lab StrainDepositorBacterial ResistanceNoneAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHyo245
Plasmid#173147PurposeExpresses pheS_A294G by dual T7 promoter in e.coliDepositorInsertpheS_A294G
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHyo182
Plasmid#173136PurposeExpresses pheS_A294G in e.coliDepositorInsertpheS_A294G
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
DH10B-M.Osp807II
Bacterial Strain#114267PurposeThe strain expresses M.Osp807II methylase and zeocin selection marker from the arsB locus.DepositorBacterial ResistanceBleocin (zeocin)Available sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
DH10B-M.XmnI
Bacterial Strain#114269PurposeThe strain expresses M.XmnI methylase and zeocin selection marker from the arsB locus.DepositorBacterial ResistanceBleocin (zeocin)Available sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only
