We narrowed to 18,577 results for: MUT

1. pRK5 TGF beta type I receptor (T202D) Flag

   Plasmid

   #14833

   Depositor

   Rik Derynck

   Article

   Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

   Insert

   TGF beta type I receptor (Tgfbr1 Rat)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Threonine 202 mutated to aspartate. Constitutivel…

   Available Since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiV_Neo_SIK3_CR

   Plasmid

   #108104

   Purpose

   Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   SIK3 (SIK3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   change guanine 501 to cytosine (silent mutation)

   Promoter

   EFS promoter

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-DU422gp140-TM

   Plasmid

   #123251

   Purpose

   Mammalian expression plasmid for soluble gp140 from the DU422 HIV-1 isolate with a transmembrane tether

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (DU422) Env
   

   Tags

   CD5 leader peptide and Linker (with 6his tag) and…

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. U6-pegRNA-H1-nick sgRNA-mCherry

   Plasmid

   #176901

   Purpose

   Expresses prime editing guide RNA and guide RNA in mammalian cells

   Depositor

   Hyongbum Kim

   Article

   Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

   Inserts

   mCherry
   gRNA scaffold
   gRNA scaffold
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG, H1, and U6

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21a Ce-iPGM-bio

   Plasmid

   #162559

   Purpose

   Expresses C. elegans iPGM containing a C-terminal biotinylation site and His tag

   Depositor

   James Inglese

   Article

   Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

   Insert

   C. elegans iPGM bio-6His (ipgm-1 Nematode)
   

   Tags

   6His tag, T7 tag, Thrombin cleavage site, and bio…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a Bm-iPGM-bio

   Plasmid

   #162565

   Purpose

   Expresses B. malayi iPGM containing a C-terminal biotinylation site and His tag

   Depositor

   James Inglese

   Article

   Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

   Insert

   B. malayi iPGM bio-6His
   

   Tags

   6His tag, T7 tag, biotinylation sequence, and thr…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHHM.X513-iNicSnFR3b-(V7)

   Plasmid

   #124881

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

   Depositor

   Henry Lester

   Article

   Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

   Insert

   iNicSnFR3b-(V7)
   

   Tags

   HA Tag, His Tag, and NA

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   T7

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV5-rat ERK2-L73P/S151D

   Plasmid

   #40819

   Depositor

   Natalie Ahn

   Article

   Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79

   Insert

   MAPK1 (Mapk1 Rat)
   

   Tags

   His6

   Expression

   Mammalian

   Mutation

   L73P, S151D

   Promoter

   CMV

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22b-T5-sfGFP*-XcP4H-MH4R

   Plasmid

   #178043

   Purpose

   Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP

   Depositor

   Han Xiao

   Article

   Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

   Inserts

   superfolder green fluorescent protein with TAG at 151 position
   XcP4H with W179F mutation
   dihydropterine reductase from human
   pterin-4a-carbinolamine dehydratase from human
   

   Tags

   His tag

   Expression

   Bacterial

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-hAR-L26A,F27A

    Plasmid

    #89084

    Purpose

    mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    hAR FxxLF mutant (AR Human)
    

    Expression

    Mammalian

    Mutation

    hAR-L26A,F27A

    Promoter

    CMV

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pCMV-AID-Ns3h-UGI

    Plasmid

    #221219

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and Ns3h helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-Ns3h-UGI
    

    Expression

    Mammalian

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR_L1-YFP-Turbo-NES-L2

    Plasmid

    #127349

    Purpose

    gateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-TEV-TBP6.9F34MF37MF77M

    Plasmid

    #168268

    Purpose

    Expresses human TBP6.9 with the F34M/F37M/F77M triple mutant

    Depositor

    Joseph Wedekind

    Article

    Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

    Insert

    TBP6.9
    

    Tags

    6xHis tag N-terminus, TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    changed F34M, F37M and F77M

    Promoter

    T7 promotor

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-hAR-F23A

    Plasmid

    #89089

    Purpose

    mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    hAR-FxxLF mutant (AR Human)
    

    Expression

    Mammalian

    Mutation

    hAR-F23A

    Promoter

    CMV

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pPB9v1

    Plasmid

    #210028

    Purpose

    Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRVL-6

    Plasmid

    #104584

    Purpose

    Contains human IgG1 CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions

    Depositor

    Daniel Christ

    Article

    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

    Insert

    Human IgG1 CH1/CH2/CH3 (mutated, no effector functions) (IGHG1 Human)
    

    Expression

    Mammalian

    Mutation

    L234A/L235E/G237A and K322A/P331S

    Promoter

    CMV

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. PTPRA-SHC

    Plasmid

    #139611

    Purpose

    StrepIII-HA-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    PTPRA (PTPRA Human)
    

    Tags

    StrepIII-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYDE009

    Plasmid

    #194151

    Purpose

    Delivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.

    Depositor

    Edward Geisinger

    Article

    Bai et al J Bacteriol. 2021 May 20;203(12):e005652

    Insert

    tetR-tetP-dcas9-rrnB T1-T7Te
    

    Expression

    Bacterial

    Promoter

    tetp

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 HA-Sumo1 Q94P

    Plasmid

    #48965

    Purpose

    Expresses HA SUMO1 Q94P mutant in mammalian cells

    Depositor

    Guy Salvesen

    Article

    Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

    Insert

    SUMO1 (SUMO1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Mutated Q94P

    Promoter

    T7

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pM9-BetaGal-8C

    Plasmid

    #172489

    Purpose

    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    M9-BetaGal-8C
    

    Tags

    6xHis and M9 nuclear localization sequence (from …

    Expression

    Bacterial

    Mutation

    BetaGal has two cysteine mutations (C77A, C1022S)…

    Promoter

    T7

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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