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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 28,145 results for: STI;

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Showing: 3021 - 3030 of 28145 results

  1. MSP977

    Plasmid
    #65774
    Purpose
    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E mutation in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65774-plasmid-map-sequence-id-112656

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  3. AICSDP-078:G3BP1-mEGFP

    Plasmid
    #193920
    Purpose
    Homology arms and mEGFP-SGLRS for N-terminus tagging of human G3BP stress granule assembly factor 1
    Depositor
    Allen Institute for Cell Science
    Insert
    G3BP1 Homology Arms with mEGFP-SGLRS (G3BP1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Dec. 22, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193920-plasmid-map-sequence-id-380523

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  5. CMV-tdTomato-P2A-paCaMKII(T286A)

    Plasmid
    #165432
    Purpose
    Expresses tdTomato and photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity.
    Depositor
    Hideji Murakoshi
    Article
    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1
    Insert
    tdTomato-P2A-paCaMKII(T286A)
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    T286A: Autophosphorylation deficient mutation
    Promoter
    CMV
    Available Since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    165432-plasmid-map-sequence-id-325984

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  9. MSP2262

    Plasmid
    #70703
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A & H557A in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70703-plasmid-map-sequence-id-127765

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Showing: 3021 - 3030 of 28145 results