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pYTSK58K_6G7

Plasmid

#177302

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and PE-H

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

pe-h

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW17K_6G5

Plasmid

#177285

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and PE-H

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

pe-h

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW13K_3G5

Plasmid

#177287

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and mCherry

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

mCherry

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW18K_3T5

Plasmid

#177288

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with TcR and mCherry

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

mCherry

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRRL-SFFV_V5-CHIC2_IFRPDdel_Tomato

Plasmid

#238126

Purpose

Expresses V5-tagged CHIC2 (with IFRPD deletion) with Tomato selectable marker.

Depositor

Arlene Sharpe

Article

LaFleur et al Nat Immunol. 2025 Sep;26(9):1476-1487. d

Insert

V5-tagged CHIC2 with IFRPD deletion

Tags

V5

Expression

Mammalian

Mutation

Deleted amino acids 160-165 (IFRPD).

Promoter

SFFV

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pRRL-SFFV_V5-CHIC2-K117R_Tomato

Plasmid

#238127

Purpose

Expresses V5-tagged CHIC2 (with K117R mutation) with Tomato selectable marker.

Depositor

Arlene Sharpe

Article

LaFleur et al Nat Immunol. 2025 Sep;26(9):1476-1487. d

Insert

V5-tagged CHIC2 with K117R mutation

Tags

V5

Expression

Mammalian

Mutation

Mutated Lysine 117 to Arginine.

Promoter

SFFV

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pRRL-SFFV_V5-CHIC2-C6S_Tomato

Plasmid

#238128

Purpose

Expresses V5-tagged CHIC2 (with C6S mutation) with Tomato selectable marker.

Depositor

Arlene Sharpe

Article

LaFleur et al Nat Immunol. 2025 Sep;26(9):1476-1487. d

Insert

V5-tagged CHIC2 with C6S mutation

Tags

V5

Expression

Mammalian

Mutation

Changed cysteines from amino acids 102-109 to ser…

Promoter

SFFV

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pRRL-SFFV_STUB1-UBOXdel-V5_Tomato

Plasmid

#238129

Purpose

Expresses V5-tagged STUB1 (with UBOX deletion) with Tomato selectable marker.

Depositor

Arlene Sharpe

Article

LaFleur et al Nat Immunol. 2025 Sep;26(9):1476-1487. d

Insert

V5-tagged-STUB1

Tags

V5

Expression

Mammalian

Mutation

Deleted UBOX domain (amino acids 224-294)

Promoter

SFFV

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-TGFa-mNeonGreen

Plasmid

#209913

Purpose

To monitor the expression of TGFα, the plasmid encodes a recombinant TGFα fused intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TGFa-mNeonGreen (TGFA Human)

Use

Lentiviral

Tags

mNeonGreen

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-DXO

Plasmid

#177993

Purpose

For purification of N-terminally His-tagged DXO from bacteria

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

DXO (DXO Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSG086

Plasmid

#214263

Purpose

PaFtsH2H1-link-32-6xHis: PaftsH2(ftsH2_F124-V154del_ins ftsH1_M120-T151)-6xHis cloned in XbaI/NheI site in pET-28a(+)

Depositor

Ute Romling

Article

Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

Insert

Hybrid PaFtsH protease PaFtsH2H1-link-32 of Pseudomonas aeruginosa SG17M

Tags

6x His-tag

Expression

Bacterial

Mutation

substitution of 31 amino acids of FtsH2 (F124RRFA…

Promoter

T7 promoter

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-miRFP670

Plasmid

#220600

Purpose

Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

miRFP670

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)

Plasmid

#220597

Purpose

Constitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mVenus-Q69M (ME)

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_Ex3_1

Plasmid

#210625

Purpose

gRNA target sequences for TAL1 Exon 3

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 Exon 3 gRNA (TAL1 Human)

Use

CRISPR

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_2

Plasmid

#210622

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_1

Plasmid

#210621

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_4

Plasmid

#210624

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_3

Plasmid

#210623

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_Ex3_2

Plasmid

#210626

Purpose

gRNA target sequences for TAL1 Exon 3

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 Exon 3 gRNA (TAL1 Human)

Use

CRISPR

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_Ex3_3

Plasmid

#210627

Purpose

gRNA target sequences for TAL1 Exon 3

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 Exon 3 gRNA (TAL1 Human)

Use

CRISPR

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge