We narrowed to 10,133 results for: ADA

1. pCMV_eA3A T31A-NG-nCas9

   Plasmid

   #163539

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A T31A-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXS-IRES-Blast HA-POLE2

   Plasmid

   #160809

   Purpose

   Express HA-tagged POLE2

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   HA-POLE2 (POLE2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN244 - CTCF-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

   Plasmid

   #92140

   Purpose

   Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using BLASTICIDIN selection. Auxin-inducible degron system.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   AID[71-114]-eGFP
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-PITCh-COX8A

   Plasmid

   #227308

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of COX8A for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of COX8A (COX8A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U67

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_ 2xFLAG-2xSTREP_FBXO22

   Plasmid

   #159127

   Purpose

   expresses FBXO22 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

   Insert

   FBXO22 (FBXO22 Human)
   

   Tags

   2X-FLAG _ 2X-STREP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVH-EF1a-GFP-P2A-KLF4

   Plasmid

   #130694

   Purpose

   Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

   Depositor

   Kejin Hu

   Article

   Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

   Insert

   human KLF4 (KLF4 Human)
   

   Use

   Lentiviral

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiCRISPR v2-sgACSL4 clone2

   Plasmid

   #162129

   Purpose

   Lentiviral sgRNA plasmid targeting human ACSL4

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgACSL4 (ACSL4 Human)
   

   Use

   Lentiviral

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiCRISPR v2-sgACSL4 clone1

   Plasmid

   #162128

   Purpose

   Lentiviral sgRNA plasmid targeting human ACSL4

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgACSL4 (ACSL4 Human)
   

   Use

   Lentiviral

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Str-KDEL_SBP-mCherry-LAMP1

   Plasmid

   #222318

   Purpose

   Synchronize the trafficking of LAMP1 from the ER.

   Depositor

   Franck Perez

   Article

   Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

   Insert

   Streptavidin-KDEL and LAMP1 fused to SBP-mCherry (LAMP1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.hSynapsin1.OxLight-ctr

    Plasmid

    #169793

    Purpose

    Control non-binding fluorescent reporter for orexin neuropeptides

    Depositor

    Tommaso Patriarchi

    Article

    Duffet et al Nat Methods. 2022 Feb;19(2):231-241. doi

    Insert

    OxLight-ctr
    

    Use

    AAV

    Mutation

    E54K; T111A

    Promoter

    human Synapsin-1

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD40-His/V5-c-MycT58A

    Plasmid

    #45598

    Depositor

    Rosalie Sears

    Article

    Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

    Insert

    c-Myc T58A (Myc Mouse)
    

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    T58A

    Promoter

    CMV

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCDH-DCX-TurboID-V5-puro

    Plasmid

    #190742

    Purpose

    A lentiviral plasmid encoding DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

    Depositor

    Jeremy Baskin

    Article

    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

    Insert

    DCX-TurboID-V5 (DCX Human, DCX is human doublecortin; TurboID is engineered BirA from E.coli)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-N-Puro-moxGFP-TUBA1B

    Plasmid

    #207767

    Purpose

    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Puro-moxGFP Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pEF1a-hMLH1dn (og codon)

    Plasmid

    #174823

    Purpose

    Mammalian expression of human MLH1dn (original codon usage)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (original codon usage) (MLH1 Human)
    

    Expression

    Mammalian

    Mutation

    deletion of residues 754-756

    Promoter

    EF1a

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-dCas12a-ABE8e-V106W

    Plasmid

    #193646

    Purpose

    Expression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-TadA*8e-V106W, EGFP
    

    Expression

    Mammalian

    Mutation

    TadA*8e-V106W

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-UBC-XRI-HA

    Plasmid

    #178056

    Purpose

    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with HA tag, under UBC promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    XRI-HA
    

    Use

    AAV

    Tags

    HA-MBP

    Expression

    Mammalian

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC1-oROS-HT-CaaX

    Plasmid

    #216417

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT-CaaX
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-GRAB_UCN1.0

    Plasmid

    #208669

    Purpose

    Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCI-neo-RL-ZEB2 200bmut

    Plasmid

    #35538

    Depositor

    Greg Goodall

    Article

    Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

    Insert

    3'UTR of ZEB2 gene (ZEB2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    6 Mutations in the miR-200b seed regions of the Z…

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. WDV-Echistatin

    Plasmid

    #226985

    Purpose

    Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

    Depositor

    Wendy Gordon

    Article

    Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

    Inserts

    WDV (repA )
    Echistatin
    

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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