We narrowed to 7,668 results for: ski

1. mCerulean-PCNA-PuroR

   Plasmid

   #215063

   Purpose

   Expresses mCerulean-PCNA in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   PCNA (PCNA Human)
   

   Use

   Retroviral

   Tags

   mCerulean

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.ZAC.GFAP.iAChSnFR

   Plasmid

   #137962

   Purpose

   Expresses iAChSnFR under GFAP promoter

   Depositor

   Loren Looger

   Article

   Borden et al bioRxiv 2020

   Insert

   iAChSnFR
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   GFAP

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX RASGAP(N)-SH2

   Plasmid

   #46432

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Gap/RASGAP(N) (RASA1 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 168-283

   Promoter

   Tac

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. Fgf4enhLV (FpG12)

   Plasmid

   #69444

   Purpose

   positive control plasmid for lentiviral reporter expression in ESCs

   Depositor

   Lisa Dailey

   Article

   Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

   Inserts

   minimal FGF4 promoter
   EGFP
   Ubiquitin promoter
   Hygromycin resistance
   FGF4 enhancer (Fgf4 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223_PCCB_WT_V5

   Plasmid

   #82938

   Purpose

   Gateway Donor vector containing PCCB, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   PCCB (PCCB Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S

   Plasmid

   #106183

   Purpose

   Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Has Service

   AAV1

   Insert

   SF-Venus-iGluSnFR.A184S
   

   Use

   AAV

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

   Promoter

   hSynapsin

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIBN-tagBFP-hTRF1

   Plasmid

   #103801

   Purpose

   BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-tagBFP-hTRF1 (CIB1, TERF1 Human, Mustard Weed)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. mKO2-CDK4

   Plasmid

   #215065

   Purpose

   Expresses mKO2-CDK4 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Retroviral

   Tags

   mKO2

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

   Plasmid

   #169336

   Purpose

   Expression of the LT-Fucci(SA) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Fucci(SA) (GMNN, CDT1 Human)
   

   Tags

   HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_SMAD4_p.P356R

    Plasmid

    #82769

    Purpose

    Gateway Donor vector containing SMAD4, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    SMAD4 (SMAD4 Human)
    

    Use

    Gateway entry vector

    Mutation

    P356R

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX SHP-2(C)-SH2

    Plasmid

    #46497

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-2(C) (PTPN11 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 101-222

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-pegRNA-SNCA-A30P

    Plasmid

    #180016

    Purpose

    Transiently expressing a pegRNA to introduce SNCA-A30P mutation in human cells

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    Prime editing pegRNA for SNCA-A30P (SNCA Synthetic)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBPK1520-SNCA-A30P-ng

    Plasmid

    #180018

    Purpose

    Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A30P mutation in PE3 approach

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    Prime editing ngRNA for SNCA-A30P (SNCA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)

    Plasmid

    #194190

    Purpose

    Includes the promoter (1kb) of SMTS with 5 mutated KLF binding sites (positions 449,548,732,899,884)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Mutation

    5 mutated KLF binding sites (positions 449,548,73…

    Promoter

    SMANTIS promoter (1kb), mutated

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3-pSMANTIS_1kb_KLF2mut 899

    Plasmid

    #194193

    Purpose

    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 899)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Mutation

    mutated KLF binding sites (position 899)

    Promoter

    SMANTIS promoter (1kb), mutated

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_POLR2I_WT_V5

    Plasmid

    #82955

    Purpose

    Gateway Donor vector containing POLR2I, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    POLR2I (POLR2I Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. CD44-isoform12-CD4d3+4-bio

    Plasmid

    #73130

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding CD44-isoform12 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    CD44 antigen isoform 12 (CD44 Human)
    

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCIBN-LacI-tagRFP-T

    Plasmid

    #103810

    Purpose

    'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-LacI-tagRFP-T (CIB1, lacI Mustard Weed, E. coli)
    

    Expression

    Mammalian

    Mutation

    LacI: C19T (silent, L7L), T264C (silent, A88A), C…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-PE SpG

    Plasmid

    #179081

    Purpose

    NOTE: An improved version of this plasmid is now available. See Addgene plasmid #242072. All-in-one prime editor piggyBac transposon, SpG variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R

    Promoter

    CAG

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMBP-parallel1-GK5_v2

    Plasmid

    #134288

    Purpose

    Expresses GK5 (isoform 2) in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    mouse Gk5 isoform 2; 534 amino acids

    Promoter

    T7

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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