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pSM038

Plasmid

#128293

Purpose

asymmetric plasmid partitioning E. coli - helper plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

PphlF - sfyfp-P2A-motA

Expression

Bacterial

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSPB866

Plasmid

#128304

Purpose

asymmetric plasmid partitioning E. coli - regulatory plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Para - GCN4zip.MSE-cfp-parB

Expression

Bacterial

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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5W9S (CXXC5)

Plasmid

#101266

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

CXXC5 (CXXC5 Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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p3xFLAG-zDrosha

Plasmid

#171540

Purpose

Encodes Zebrafish Drosha protein

Depositor

Yan Zeng

Article

Li et al Biochem Biophys Res Commun. 2019 Jun 18;

Insert

drosha (rnasen Zebrafish)

Expression

Mammalian

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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NTERY06

Plasmid

#184124

Purpose

Nanopore-addressable protein reporter for expression in bacterial cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

NTERY06

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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mNTERY00

Plasmid

#184110

Purpose

Nanopore-addressable protein reporter for expression in mammalian cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

mNTERY00

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pRS416-ACT1_intron

Plasmid

#127602

Purpose

low-copy URA-selectable plasmid, ACT1 intron inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ACT1 intron

Expression

Yeast

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCC48

Plasmid

#128285

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq - mRFP, BBa_J23100 - phlF, parS

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS426-ACT_unstable

Plasmid

#127608

Purpose

high-copy URA-selectable plasmid, WT ACT1 intron (unstable) inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

Unstable ACT1 intron (WT)

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS426-ECM_stable

Plasmid

#127606

Purpose

high-copy URA-selectable plasmid, WT ECM33 intron (stable) inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

Stable ECM33 intron (WT)

Expression

Yeast

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pNQ116

Plasmid

#242080

Purpose

Expresses NusG (M1-P124) as fusion with 6xHis-SUMO

Depositor

Olivier Duss

Article

Qureshi et al Nature. 2025 Jan;637(8045):487-495. doi:

Insert

NusG (nusG E. coli)

Tags

SUMO

Expression

Bacterial

Mutation

conatins M1-P124

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pNQ117

Plasmid

#242081

Purpose

Expresses NusG-F165A as fusion with 6xHis-SUMO

Depositor

Olivier Duss

Article

Qureshi et al Nature. 2025 Jan;637(8045):487-495. doi:

Insert

NusG (nusG E. coli)

Tags

SUMO

Expression

Bacterial

Mutation

F165A

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pNQ055

Plasmid

#242077

Purpose

Expresses MetRS (truncated at K548) as fusion with 6xHis-SUMO

Depositor

Olivier Duss

Article

Qureshi et al Nature. 2025 Jan;637(8045):487-495. doi:

Insert

metG (metG E. coli)

Tags

SUMO

Expression

Bacterial

Mutation

contains M1-K548

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pNQ056

Plasmid

#242078

Purpose

Expresses MTF as fusion with 6xHis-SUMO

Depositor

Olivier Duss

Article

Qureshi et al Nature. 2025 Jan;637(8045):487-495. doi:

Insert

FMT (fmt E. coli)

Tags

SUMO

Expression

Bacterial

Mutation

M1 is deleted

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-DARPin-SpyCatcher

Plasmid

#243754

Purpose

For affinity purification of E01 anti-EGFR DARPin fused to SpyCatcher

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

DARPin-SpyCatcher

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-Nb-SpyCatcher

Plasmid

#243752

Purpose

For affinity purification of anti-mouse MHC-II nanobody (Nb) fused to SpyCatcher

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

Nb-SpyCatcher

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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TwinStrepSUMO-scFv-SpyCatcher

Plasmid

#243753

Purpose

For affinity purification of anti-HA scFv fused to SpyCatcher

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

scFv-SpyCatcher

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-SNAP

Plasmid

#243741

Purpose

PVC structural operon - tail fiber (Pvc13) tagged with SNAP tag

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-SNAP

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-SpyTag

Plasmid

#243739

Purpose

PVC structural operon - tail fiber (Pvc13) tagged with SpyTag

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-SpyTag

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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QBEn

Plasmid

#238002

Purpose

Expresses the QBEn base editor in mammalian cells

Depositor

Kevin Zhao

Article

Hu et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

QBEn base editor

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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