We narrowed to 7,668 results for: ski

1. pDONR223_STC2_WT

   Plasmid

   #82883

   Purpose

   Gateway Donor vector containing STC2, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   STC2 (STC2 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEFL GAL4dbd-KLF4 1-329

   Plasmid

   #140146

   Purpose

   GAL4-DNA binding domain fusion protein with the 329 N-terminal amino acids of human KLF4

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

   Insert

   KLF4 Kruppel factor (KLF4 Human)
   

   Tags

   GAL4dbd

   Expression

   Mammalian

   Mutation

   Deleted amino acids 330 to 479

   Promoter

   EF1a

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_STC2_WT_V5

   Plasmid

   #82957

   Purpose

   Gateway Donor vector containing STC2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   STC2 (STC2 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR223_RBM45_WT_V5

   Plasmid

   #82968

   Purpose

   Gateway Donor vector containing RBM45, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   RBM45 (RBM45 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEFL GAL4dbd-KLF4 1-156

   Plasmid

   #140147

   Purpose

   GAL4-DNA binding domain fusion protein with the 156 N-terminal amino acids of human KLF4 (activation domain)

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

   Insert

   KLF4 aa 1-156 (KLF4 Human)
   

   Tags

   GAL4dbd

   Expression

   Mammalian

   Mutation

   Deleted amino acids 157 to 479

   Promoter

   EF1a

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABE-hygro-BAP1-G312G-HA

   Plasmid

   #154022

   Purpose

   Mammalian Expression of HA-tagged BAP1 with the Synonymous Mutation p.G312G

   Depositor

   Samuel Peña-Llopis

   Article

   Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

   Insert

   BAP1 (BAP1 Human)
   

   Use

   Retroviral

   Tags

   Hemagglutinin (HA)

   Expression

   Mammalian

   Mutation

   c.936T>G, p.G312G (Synonymous Mutation) by Sit…

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_PSMD9_WT_V5

   Plasmid

   #82950

   Purpose

   Gateway Donor vector containing PSMD9, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   PSMD9 (PSMD9 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_NOSIP_WT_V5

   Plasmid

   #82970

   Purpose

   Gateway Donor vector containing NOSIP, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   NOSIP (NOSIP Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.S72A

   Plasmid

   #106185

   Purpose

   Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Has Service

   AAV1

   Insert

   SF-Venus-iGluSnFR.S72A
   

   Use

   AAV

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

   Promoter

   hSynapsin

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCC_07 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid

    #139092

    Purpose

    Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    dxCas9 3.7-VPR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX RASGAP(C)-SH2

    Plasmid

    #46431

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Gap/RASGAP(C) (RASA1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 339-452

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO-Tet-On-PLXNB2-shRNA2

    Plasmid

    #98400

    Purpose

    Lentivirus for expression of shRNA2 against human PLEXIN-B2 (Dox-inducible)

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    Plexin-B2 (PLXNB2 Human)
    

    Use

    Lentiviral

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223_BRP44_WT_V5

    Plasmid

    #82976

    Purpose

    Gateway Donor vector containing BRP44, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    MPC2 (MPC2 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX RASGAP(NC)-SH2

    Plasmid

    #46433

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Gap/RASGAP(NC) (RASA1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains amino acids 171-448

    Promoter

    Tac

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. W118-1_hRSK3(RPS6KA2)_K91A_K444A

    Plasmid

    #180385

    Purpose

    lentiviral transduction of human RSK3 (RPS6KA2) gene with point mutations (K91A and K444A) that inactivate kinase activity

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    RPS6KA2_K91A/K444A (RPS6KA2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    K91A/K444A

    Promoter

    CMV

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_RBM45_p.D434Y

    Plasmid

    #82758

    Purpose

    Gateway Donor vector containing RBM45 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    RBM45 (RBM45 Human)
    

    Use

    Gateway entry vector

    Mutation

    D434Y

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223_RBM45_p.M126I

    Plasmid

    #82759

    Purpose

    Gateway Donor vector containing RBM45 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    RBM45 (RBM45 Human)
    

    Use

    Gateway entry vector

    Mutation

    M126I

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX

    Plasmid

    #112762

    Purpose

    express GFP-4EBP1-Rheb15(CAAX mutation)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-GFP-4EBP1-Rheb15 (CAAX mutant) (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Rheb15(CAAX mutant)

    Mutation

    CAAX mutation to prevent lipid localization

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRCN0000297275

    Plasmid

    #78152

    Purpose

    shCDK4 targeting 3UTR

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    CDK4 (CDK4 Human)
    

    Use

    RNAi

    Promoter

    hU6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pet-ECHB(35-475)

    Plasmid

    #96890

    Purpose

    expresses His-tagged mouse ECHB (35-475) in E.coli cells

    Depositor

    Hening Lin

    Article

    Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

    Insert

    Trifunctional enzyme subunit beta, mitochondrial (Hadhb Mouse)
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-34

    Promoter

    T7

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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