We narrowed to 10,155 results for: ADA

1. pCIneoMyc UNC119Ab

   Plasmid

   #128332

   Purpose

   Mammalian expression vector of Myc-tagged human UNC119A isoform b

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

   Insert

   UNC119 (UNC119 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-RR-ABE8e-V106W

   Plasmid

   #193656

   Purpose

   Expression of base editor RR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-TadA*8e-V106W, EGFP
   

   Expression

   Mammalian

   Mutation

   dLbCas12a-G532R/K595R, TadA*8e-V106W

   Promoter

   CMV

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDisplay-GRAB_CCK1.0

   Plasmid

   #208672

   Purpose

   Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT2-TetR-neoR

   Plasmid

   #153310

   Purpose

   Tet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GW

   Depositor

   Spyros Petrakis

   Article

   Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

   Insert

   TetR
   

   Use

   Transposon

   Promoter

   SV40

   Available Since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-miRFP-6xFLAG

   Plasmid

   #161614

   Purpose

   Cell morphology marker construct. Contains a near infrared fluorescent protein miRFP and six FLAG tags.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   miRFP-6xFLAG
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human synapsin 1 promoter

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V

   Plasmid

   #119723

   Purpose

   AAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Thomas Oertner

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Inserts

   CaMPARI2
   CaMPARI2
   

   Use

   AAV

   Tags

   NES-his

   Expression

   Mammalian

   Mutation

   F391W, L398V

   Promoter

   hSyn1

   Available Since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDB-hPER2-mScarlet-I-CD4-bla

   Plasmid

   #179449

   Purpose

   Donor vector to knock in mScarlet-I C-terminal to human PER2 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   mScarletI
   

   Use

   CRISPR

   Tags

   His/Flag

   Promoter

   None

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNOC_hlbCas12a-Nlux

   Plasmid

   #176240

   Purpose

   pNOC episomal plasmid harboring the humanized lbCas12a gene sequence tagged with Nlux under the control of Ribi promoter

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized lbCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Promoter

   Ribosomal subunit

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-mRuby3-6xFLAG

   Plasmid

   #161613

   Purpose

   Cell morphology marker construct. Contains a red fluorescent protein mRuby3 and six FLAG tags.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   mRuby3-6xFLAG
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human synapsin 1 promoter

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNOC_hfnCas12a_NR1_NR2_LPT1

    Plasmid

    #176253

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene and one spacer targeting the LPAAT gene

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-N-Puro-sTagRFP-TUBA1B

    Plasmid

    #207769

    Purpose

    Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. Myh6-Cas9

    Plasmid

    #109037

    Purpose

    Cardiac-specific overexpression of Cas9

    Depositor

    Eva van Rooij

    Article

    Johansen et al Circ Res. 2017 Oct 27;121(10):1168-1181.

    Insert

    Cas9
    

    Tags

    3XFlag

    Expression

    Mammalian

    Promoter

    alpha MHC

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGLO-GFP-3UAG

    Plasmid

    #82501

    Purpose

    Expresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Green Fluorescence Protein with 3 UAG codons
    

    Expression

    Bacterial

    Mutation

    Added an UAG codon at the amino acid position 3, …

    Promoter

    AraBAD

    Available Since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. W118-1_Flag-hIRAK1

    Plasmid

    #180405

    Purpose

    lentiviral transduction of human IRAK1 (with an N-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    IRAK1 (IRAK1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLL5.0-Anillin shRNA

    Plasmid

    #187270

    Purpose

    Lentiviral expression of shRNA targeting Anillin

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    shRNA for Anillin (ANLN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    U6

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKK-TEV-mClover3

    Plasmid

    #105795

    Purpose

    Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mClover3

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pD40-His/V5-c-MycS62A

    Plasmid

    #45599

    Depositor

    Rosalie Sears

    Article

    Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

    Insert

    c-Myc S62A (Myc Mouse)
    

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    S62A

    Promoter

    CMV

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-hSyn-EGFP-CAAX

    Plasmid

    #208684

    Purpose

    Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    membrane-localized enhanced green fluorescent protein EGFP-CAAX
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcsII-pb4-flag

    Plasmid

    #86768

    Purpose

    C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    proteasome beta subunit 4 (PSMB4 Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKK-TEV-mVenus

    Plasmid

    #105791

    Purpose

    Expression of your protein of interest in fusion with yellow fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mVenus

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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