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pCS2+8-cyp-33e1(C295A)-flag-tev-halo-his

Plasmid

#227156

Purpose

For T-REX experiments of cyp-33e1 C295A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 295 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1(C439A)-flag-tev-halo-his

Plasmid

#227157

Purpose

For T-REX experiments of cyp-33e1 C439A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 439 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_CaMKII-RFP-p2A-DAAO(R285A)-NES

Plasmid

#238919

Purpose

Mammalian expression of mutated DAAO(R285A) with a nuclear export signal and RFP as a reporter in excitatory neurons

Depositor

Vsevolod Belousov

Article

Kalinichenko et al Redox Biol. 2023 Apr;60:102604. doi: 10.

Insert

RFP-p2A-DAAO(R285A)-NES

Use

AAV

Expression

Mammalian

Promoter

CaMKIIalfa

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_CMV-RFP-p2A-DAAO(R285A)-NES

Plasmid

#238921

Purpose

Expression of mutated DAAO(R285A) with a nuclear export signal and RFP as a reporter in mammalian cells

Depositor

Vsevolod Belousov

Article

Kalinichenko et al Redox Biol. 2023 Apr;60:102604. doi: 10.

Insert

RFP-p2A-DAAO(R285A)-NES

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHIV-IL13-TetCoil

Plasmid

#240244

Purpose

Lentiviral transfer plasmid encoding IL13 on tetrameric coiled coil

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

IL-13 (IL13 Human)

Use

Lentiviral

Tags

EGFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

July 18, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV41

Plasmid

#237995

Purpose

Encodes TRAV41 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV41 (TRAV41 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV27

Plasmid

#237985

Purpose

Encodes TRAV27 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV27 (TRAV27 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV7-3

Plasmid

#237922

Purpose

Encodes TRBV7-3 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV7-3-TRAC (TRBV7-3 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV6-2

Plasmid

#237914

Purpose

Encodes TRBV6-2 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV6-2-TRAC (TRBV6-2 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TRBV6-1

Plasmid

#237913

Purpose

Encodes TRBV6-1 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV6-1-TRAC (TRBV6-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV21

Plasmid

#237978

Purpose

Encodes TRAV21 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV21 (TRAV21 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV10-2

Plasmid

#237930

Purpose

Encodes TRBV10-2 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV10-2-TRAC (TRBV10-2 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV6

Plasmid

#237957

Purpose

Encodes TRAV6 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV6 (TRAV6 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV7

Plasmid

#237958

Purpose

Encodes TRAV7 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV7 (TRAV7 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV6-9

Plasmid

#237920

Purpose

Encodes TRBV6-9 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV6-9-TRAC (TRBV6-9 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV6-5

Plasmid

#237917

Purpose

Encodes TRBV6-5 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV6-5-TRAC (TRBV6-5 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV3

Plasmid

#237954

Purpose

Encodes TRAV3 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV3 (TRAV3 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TRBV4-1

Plasmid

#237905

Purpose

Encodes TRBV4-1 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV4-1-TRAC (TRBV4-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV3-1

Plasmid

#237904

Purpose

Encodes TRBV3-1 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV3-1-TRAC (TRBV3-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-sfGFP-TurboID-ER

Plasmid

#240230

Purpose

Expression of ER-localized sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

BIP-sfGFP-TurboID-KDEL

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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