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(P6)RBSS4

Plasmid

#251389

Purpose

RBS from photosystem II D1 gene psbA2; slr1311 (Synechocystis)

Depositor

Kate Adamala, Pauli Kallio

Article

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

RBS S4

Expression

Bacterial

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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(P2)RBSB

Plasmid

#251373

Purpose

RBS designed in silico [75] for high expression in E. coli

Depositor

Kate Adamala, Pauli Kallio

Article

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

RBS B

Expression

Bacterial

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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(P2)RBSS4

Plasmid

#251369

Purpose

RBS from photosystem II D1 gene psbA2; slr1311 (Synechocystis)

Depositor

Kate Adamala, Pauli Kallio

Article

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

RBS S4

Expression

Bacterial

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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(P6)RBSB

Plasmid

#251393

Purpose

RBS designed in silico [75] for high expression in E. coli

Depositor

Kate Adamala, Pauli Kallio

Article

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

RBS B

Expression

Bacterial

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pDonor RUNX3-P2A-tdTomato

Plasmid

#246655

Purpose

Donor plasmid for Crispr/Cas9 gene editing of the human RUNX3 locus

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

P2A-tdTomato flanked by RUNX3 homology regions (RUNX3 Human, Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pDonor MAFA-P2A-tdTomato

Plasmid

#246654

Purpose

Donor plasmid for Crispr/Cas9 gene editing of the human MAFA locus

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

P2A-tdTomato flanked by MAFA homology regions (MAFA Human, Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pDonor NTRK1-P2A-tdTomato

Plasmid

#246653

Purpose

Donor plasmid for Crispr/Cas9 gene editing of the human NTRK1 locus

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

P2A-tdTomato flanked by NTRK1 homology regions (NTRK1 Human, Synthetic)

Use

CRISPR

Expression

Mammalian

Mutation

tdTomato was altered to contain a silent SAL1 res…

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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CBE-Cas12a-GFP

Plasmid

#246533

Purpose

Plant co-editing vector

Depositor

Nian Wang

Article

Huang et al Nat Plants. 2023 Oct;9(10):1591-1597. do

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

CmYLCV

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_H0-ABD+

Plasmid

#234552

Purpose

CTNNA1 enhanced-actin-binding domain mutations

Depositor

Cara Gottardi

Article

Quinn et al Mol Biol Cell. 2024 May 1;35(5):ar65. do

Insert

mEGFP:hCTNNA1_H0-ABD+ (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

R666G, A667S, I668G, M669S

Promoter

CMV

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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Cux1-SE(IRS)-eGFP-PGK-H2BRFP

Plasmid

#241866

Purpose

Inner root sheath (IRS)-specific Cux1 epicenter-driven fluorescent reporter

Depositor

Elaine Fuchs

Article

Adam et al Nature. 2015 May 21;521(7552):366-70. do

Insert

Cux1-SE(IRS)-eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Cux1-SE(HS)-eGFP-PGK-H2BRFP

Plasmid

#241867

Purpose

Hair shaft (HS)-specific Cux1 epicenter-driven fluorescent reporter

Depositor

Elaine Fuchs

Article

Adam et al Nature. 2015 May 21;521(7552):366-70. do

Insert

Cux1-SE(HS)-eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PB-U6-CNCB_sgPOU5F1

Plasmid

#242174

Purpose

A piggybac-based vector containing mouse U6 promoter-driven POU5F1sgRNA and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

Depositor

Kazutoshi Takahashi

Article

Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

Insert

POU5F1 (POU5F1 Human)

Use

Piggybac

Expression

Mammalian

Promoter

mouse U6 promoter

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MIGR1-U6gRNA1-Filler-v1

Plasmid

#237399

Purpose

For inserting a single guide RNA or later cloning two U6-gRNA cassettes with MIGR1-U6gRNA1-Filler-v2.

Depositor

Zuoming Sun

Article

Zhong et al STAR Protoc. 2025 Jun 20;6(2):103831. do

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6, PGK

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_M-Open-DDD

Plasmid

#234561

Purpose

CTNNA1 M2-M3 salt-bridge disrupting mutant

Depositor

Cara Gottardi

Article

Quinn et al Mol Biol Cell. 2024 May 1;35(5):ar65. do

Insert

mEGFP:hCTNNA1_M-Open-DDD (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

D392A, D500A, D503A

Promoter

CMV

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mEGFP-hCTNNA1_M-Open-KRR

Plasmid

#234562

Purpose

CTNNA1 M2-M3 salt-bridge disrupting mutant

Depositor

Cara Gottardi

Article

Quinn et al Mol Biol Cell. 2024 May 1;35(5):ar65. do

Insert

mEGFP:hCTNNA1_M-Open-KRR (CTNNA1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

K525A, R548A, R551A

Promoter

CMV

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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PB-2G-CNCB

Plasmid

#236764

Purpose

A piggybac-based cloning vector containing dual sgRNAs for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

Depositor

Kazutoshi Takahashi

Article

Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

Type

Empty backbone

Use

Piggybac

Expression

Mammalian

Promoter

mouse U6 promoter, human U6 promoter, CAG promoter

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG p85beta

Plasmid

#237336

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Cell Biol. 2013 May;15(5):472-80. do

Insert

p85beta (PIK3R2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-K.l.LEU2

Plasmid

#229229

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-TRP1

Plasmid

#229228

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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MPC1-QQ-FLAG

Plasmid

#228905

Purpose

Expression of mouse MPC1 in which K45 and K46 motif sites were replaced with glutamine to mimic acetylated lysines with a C terminal FLAG tag

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

Insert

MPC1 (Mpc1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

K45 and K46 motif sites were both replaced with g…

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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