We narrowed to 15,187 results for: pET

1. pECFP-Rac1Y40C

   Plasmid

   #105293

   Purpose

   Control for FRET biosensor analysis - cannot bind downstream effectors

   Depositor

   Kenneth Yamada

   Article

   Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

   Insert

   Rac1 (RAC1 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Y40C

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PLEX_313 GFP

   Plasmid

   #184494

   Purpose

   Lentivirus expression for GFP

   Depositor

   Todd Golub, Peter Tsvetkov

   Article

   Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

   Insert

   GFP
   

   Use

   Lentiviral

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. CapChR2_pUAST-attb

   Plasmid

   #188041

   Purpose

   Expresses CapChR2 in Drosophila

   Depositor

   Peter Hegemann

   Article

   Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

   Insert

   CapChR2
   

   Tags

   none

   Expression

   Insect

   Mutation

   S43D, L112C, T139C and N238E mutations in CoChR

   Promoter

   UAS

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCytERM-hfYFP

   Plasmid

   #186520

   Purpose

   Visualization of the ER in cells using hyperfolder YFP (compatible with OSER assay)

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Hyperfolder YFP
   

   Tags

   Cytochrome p450 first 29 animo acids (Oryctolagus…

   Expression

   Mammalian

   Mutation

   mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPO635

   Plasmid

   #195297

   Purpose

   pACYC-plac-MycaTnsABC, lac promoter expressing transposase genes TnsABC of McCAST (Tn7575).

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   TnsABC operon of McCAST (Tn7575)
   

   Expression

   Bacterial

   Promoter

   IPTG inducible lac promoter

   Available Since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSYN-VWF-039

   Plasmid

   #120292

   Purpose

   VWF expression construct expressing D'D3 domains with C1099A/C1142A mutations in D3 domain as an Fc fusion protein

   Depositor

   Robert Peters

   Article

   Dong et al Blood. 2019 Jan 14. pii: blood-2018-10-8

   Insert

   VWF D1-D2-D'-D3-Fc
   

   Tags

   Fc

   Expression

   Mammalian

   Mutation

   C1099A/C1142A

   Promoter

   CMV

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPO636

   Plasmid

   #195299

   Purpose

   pACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes TnsABCQ of McCAST (Tn7575).

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   TnsABCQ operon of McCAST (Tn7575)
   

   Expression

   Bacterial

   Promoter

   IPTG inducible lac promoter

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pECFP-Cdc42-Y40C

   Plasmid

   #105295

   Purpose

   Control for FRET biosensor analysis - cannot bind downstream effectors

   Depositor

   Kenneth Yamada

   Article

   Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

   Insert

   Cdc42 (CDC42 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Y40C

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOCC110

   Plasmid

   #118905

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C, and C-terminal sortase site (LPETGg)

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-NotI-ccdB-AscI-LPETGg-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease and LPETGg

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKG3666_pGEEC576_pShip-EFS-ZF43a(inducible)-P2A-iRFP720-bGH

    Plasmid

    #239740

    Purpose

    Plasmid expressing a rapamycin-inducible transcriptional activator

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720
    

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJB65

    Plasmid

    #226319

    Purpose

    E. coli protein expression for protein purification

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    6xHis- (VTG to AAA MR1-6)-strep
    

    Tags

    6xHis and Strep

    Expression

    Bacterial

    Mutation

    N-terminal domain deleted, C-terminal domain dele…

    Promoter

    T7

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBDP

    Plasmid

    #217827

    Purpose

    Stuffer plasmid with golden gate compatible bidirectional promoter

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Insert

    Gal1/Gal10 Bidirectional Promoter
    

    Use

    Cloning vector

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. eSrtA-MT4

    Plasmid

    #200306

    Purpose

    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

    Insert

    eSrtA-LPETG-MT4 (MT4 Human)
    

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pnEA-NpM-Hs4EBP1_50-83-GB1_AA

    Plasmid

    #148275

    Purpose

    Bacterial Expression of Hs4EBP1_50-83

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    Hs4EBP1_50-83 (EIF4EBP1 Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pnEA-NpM-Hs4EBP1_50-83-L75AV81A-GB1_AA

    Plasmid

    #148276

    Purpose

    Bacterial Expression of Hs4EBP1_50-83-L75AV81A

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    Hs4EBP1_50-83-L75AV81A (EIF4EBP1 Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pnEA-NpM-Hs4EBP1_50-83-Y54AL59A-GB1_AA

    Plasmid

    #148277

    Purpose

    Bacterial Expression of Hs4EBP1_50-83-Y54AL59A

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    Hs4EBP1_50-83-Y54AL59A (EIF4EBP1 Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pnEA-NpM-Hs4EBP1_50-83-YLLVtoAAAA-GB1_AA

    Plasmid

    #148278

    Purpose

    Bacterial Expression of Hs4EBP1_50-83-YLLVtoAAAA

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    Hs4EBP1_50-83-YLLVtoAAAA (EIF4EBP1 Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pnEA-NpM-Hs4EBP3_36-67-GB1_AA

    Plasmid

    #148279

    Purpose

    Bacterial Expression of Hs4EBP3_36-67

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    Hs4EBP3_36-67 (EIF4EBP3 Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pnYC-NpH-HseIF4E_36-217_AA

    Plasmid

    #148282

    Purpose

    Bacterial Expression of HseIF4E_36-217

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    HseIF4E_36-217 (EIF4E Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pnEA-NpM-DmCUP_325-376-R329E-GB1_AA

    Plasmid

    #148263

    Purpose

    Bacterial Expression of DmCup_325-376-R329E

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    DmCup_325-376-R329E (cup Fly)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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