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Showing: 601 - 620 of 33581 results

  1. pdCas9-bacteria

    Plasmid
    #44249
    Purpose
    aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dCas9 (bacteria)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    pLtetO-1
    Available since
    April 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    44249-plasmid-map-sequence-id-269588

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  2. dCas9 plasmid

    Plasmid
    #100091
    Purpose
    dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    3XFlag-NLS-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100091-plasmid-map-sequence-id-197565

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  6. lentiCRISPR v2-dCas9

    Plasmid
    #112233
    Purpose
    Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2
    Depositor
    Thomas Gilmore
    Article
    Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do
    Inserts
    dCas9
    Porumycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Changed: Aspartic acid 10 to Glycine, Histidine 8…
    Promoter
    EFS-NS
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112233-plasmid-map-sequence-id-213540

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  8. dCas9-dMSK1

    Plasmid
    #165602
    Purpose
    Expresses Sp dCas9 fused to truncated human MSK1 (42-802)
    Depositor
    Isaac Hilton
    Article
    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1
    Insert
    S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 Human, Synthetic, S. Pyogenes)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    165602-plasmid-map-sequence-id-327024

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  16. pSCAR_Cas9-blast_GFP

    Plasmid
    #162074
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-blasticidin S deaminase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162074-plasmid-map-sequence-id-471574

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  18. eSpCas9(1.1)

    Plasmid
    #71814
    Purpose
    Expresses high specificity SpCas9. Px330-like plasmid.
    Depositor
    Feng Zhang
    Article
    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.
    Has Service
    Cloning Grade DNA
    Insert
    enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71814-plasmid-map-sequence-id-177023

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Showing: 601 - 620 of 33581 results