We narrowed to 845 results for: Cerulean

1. pcDNA3-AktAR

   Plasmid

   #61624

   Purpose

   FRET-based biosensor for monitoring Akt activity

   Depositor

   Jin Zhang

   Article

   Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

   Insert

   AktAR (AKT1 Budding Yeast, Human, Synthetic)
   

   Tags

   Cerulean and cpVenus[E172]

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-AMPKsub-YPet-NES

   Plasmid

   #84635

   Purpose

   Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   AMPKsub-YPet-NES
   

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-PKCsub-YPet-NES

   Plasmid

   #138207

   Purpose

   Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKCsub-YPet-NES
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-JNKsubDD-YPet-NES

   Plasmid

   #84633

   Purpose

   Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   JNKsubDD-YPet-NES
   

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-PKAsub-YPet-NES

   Plasmid

   #138203

   Purpose

   Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKAsub-YPet-NES
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-PKAsub-YPet-Kras

   Plasmid

   #138206

   Purpose

   Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKAsub-YPet-Kras
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-TORCAR

   Plasmid

   #64927

   Purpose

   FRET-based biosensor for monitoring mTORC1 kinase activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   TORCAR (EIF4EBP1 Human, Synthetic)
   

   Tags

   Cerulean and YPet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-TORCAR(T/A)

   Plasmid

   #64928

   Purpose

   Negative-control mutant for TORCAR biosensor.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   TORCAR(T/A) (EIF4EBP1 Human, Synthetic)
   

   Tags

   Cerulean and YPet

   Expression

   Mammalian

   Mutation

   4EBP1 domain contains T37A and T46A mutations.

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. SiC-V2-Cas9G7

   Plasmid

   #133043

   Purpose

   Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.

   Depositor

   Sriram Neelamegham

   Article

   Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

   Insert

   Tet repressor
   

   Use

   Lentiviral

   Tags

   Cerulean

   Available Since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Rap1B FLARE

    Plasmid

    #134927

    Purpose

    Rap1B biosensor

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    RaIGDS RBD, YPet, Cerulean, Rap1B
    

    Expression

    Mammalian

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLN410 (FuGW-S(E2F1)p-ECFP sponge control)

    Plasmid

    #105182

    Purpose

    Module 2 control - synthetic promoter E2F1 drives ECFP only (no miR1 binding sites)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(E2F1)p-ECFP (Cerulean) sponge control
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-PKCsub(T/A)-YPet-NES

    Plasmid

    #138208

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKCsub(T/A)-YPet-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    Sept. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-AMPKsub-YPet-Kras

    Plasmid

    #84638

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub-YPet-Kras
    

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-PKAsub(T/A)-YPet-NES

    Plasmid

    #138205

    Purpose

    Encodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKAsub(T/A)-YPet-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Lyso-TORCAR

    Plasmid

    #64929

    Purpose

    Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    LAMP1-TORCAR (LAMP1 Human, Synthetic)
    

    Tags

    Cerulean, Full-length LAMP1, and YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-TORCAR-NLS

    Plasmid

    #64931

    Purpose

    Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR-NLS (EIF4EBP1 Human, Synthetic)
    

    Tags

    Cerulean, Nuclear localization signal (NLS), and …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-PM-TORCAR

    Plasmid

    #64930

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    Lyn-TORCAR (EIF4EBP1 Human, Synthetic)
    

    Tags

    Cerulean, N-terminal targeting sequence from Lyn …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-AMPKsub(TA)-YPet-NES

    Plasmid

    #84636

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub(TA)-YPet-NES
    

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-JNKsub(TA)DD-YPet-NES

    Plasmid

    #84634

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    JNKsub(TA)DD-YPet-NES
    

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AI-CN590

    Plasmid

    #62596

    Purpose

    KCTD13 N-terminus disruption by homolgy-directed repair

    Depositor

    Joshua Grimley

    Article

    Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

    Insert

    Cerulean-P2A-PuroR (KCTD13 Human)
    

    Use

    TALEN; Homology-directed repair donor

    Expression

    Mammalian

    Promoter

    hEF1alpha

    Available Since

    March 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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