We narrowed to 886 results for: nanobody

1. pUASp-GBP-Tbro

   Plasmid

   #179995

   Purpose

   Expresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASp version is ideal for expression in the germline.

   Depositor

   Graydon Gonsalvez

   Article

   Baker et al Development. 2021 Nov 15;148(22). pii: 2

   Insert

   GBP nanobody
   

   Tags

   Trbo

   Expression

   Insect

   Promoter

   UAS-Gal4

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSb_init containing the MBP sybody Sb_MBP#1

   Plasmid

   #132699

   Purpose

   expresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISA

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   sybody_MBP#1
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMyosin-miRFP670nano3

   Plasmid

   #184668

   Purpose

   LC-Myosin labelled with near-infrared fluorescent protein miRFP670nano3

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   miRFP670nano3-LC-Myosin
   

   Tags

   miRFP670nano3

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. LgBit-NbALFA

   Plasmid

   #201484

   Purpose

   NanoBit detection of ALFA-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   LgBit-NbALFA
   

   Tags

   LgBit

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTP341 pHAGE2 CMV TagBFP-3xFLAG

   Plasmid

   #199391

   Purpose

   Lentiviral transfer plasmid for constitutive expression of a TagBFP-3xFLAG in mammalian cells

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   TagBFP-3xFLAG
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET51b(+)_ALFA-tag-SNAP

   Plasmid

   #136628

   Purpose

   Plasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusion

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   EGFP-NbALFA
   

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNIR-FbGFPY58/C-PKI

   Plasmid

   #184685

   Purpose

   NIR-Fb-based PKA inhibitor

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-FbGFPY58/C-PKI
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. NbAlfa-LgBit

   Plasmid

   #201483

   Purpose

   NanoBit detection of ALFA-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   NbALFA-LgBit
   

   Tags

   LgBit

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS

   Plasmid

   #199346

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3.0_nucLAMA-G97 IRES EGFP

    Plasmid

    #136620

    Purpose

    Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-G97-NLS (IRES EGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Gal4DBD-GBP2

    Plasmid

    #49439

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP2-Gal4 DNA binding domain fusion protein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2 PCNA GFP

    Plasmid

    #105937

    Purpose

    in vitro transcription, cell cycle nuclear marker

    Depositor

    Caren Norden

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    PCNA (PCNA Human)
    

    Tags

    GFP

    Expression

    Bacterial

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2+_Flag-myc-NES-Tir1

    Plasmid

    #117717

    Purpose

    mRNA synthesis of Flag-myc-NES-TIR1 for injection

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    TRANSPORT INHIBITOR RESPONSE 1
    

    Tags

    Flag-Myc-NES

    Expression

    Mammalian

    Mutation

    codon optimized TIR1 for murine expression

    Promoter

    SP6

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTP924 pHAGE2 CMV GFP

    Plasmid

    #199392

    Purpose

    Lentiviral transfer plasmid for constitutive expression of a GFP in mammalian cells

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-p65AD-GBP6

    Plasmid

    #49440

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP6-p65 transactivation domain fusion protein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDV5 containing Sb-convex

    Plasmid

    #132696

    Purpose

    pRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curve

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    Sb_convex including 5' and 3' flanking sequences used for ribosome display
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

    Plasmid

    #199356

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCOLA-Spec-yibDp-Erv1p

    Plasmid

    #212094

    Purpose

    Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    Sulfydryl oxidase (ERV1 Budding Yeast)
    

    Expression

    Bacterial

    Promoter

    yibDp

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-GBP6-10gly-VPminx4

    Plasmid

    #50796

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP6-VPminx4 activation domain fusion protein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-rTetRDBD-GBP1

    Plasmid

    #50791

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    rTetR DNA binding domain-GBP1 fusion protein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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