Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 990 results for: nanobody

Showing: 601 - 620 of 990 results

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pET21-pelB-LaG-14

Plasmid

#172745

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-14

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-L-IGHJ4_2-His

Plasmid

#233335

Purpose

To express nanobody IGHJ4_2 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-IGHJ4_2-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-L-IGHJ4_3-His

Plasmid

#233336

Purpose

To express nanobody IGHJ4_3 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-IGHJ4_3-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-L-IGHJ4_4-His

Plasmid

#233337

Purpose

To express nanobody IGHJ4_4 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-IGHJ4_4-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-L-IGHJ5-His

Plasmid

#233338

Purpose

To express nanobody IGHJ5 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-IGHJ5-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_C6-Cys-His

Plasmid

#233323

Purpose

To express nanobody C6 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

C6-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_VHH16-Cys-His

Plasmid

#233324

Purpose

To express nanobody VHH16 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

VHH16-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_VHH21-Cys-His

Plasmid

#233325

Purpose

To express nanobody VHH21 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

VHH21-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_1-Cys-His

Plasmid

#233326

Purpose

To express nanobody IGHJ4_1 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_1-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_2-Cys-His

Plasmid

#233327

Purpose

To express nanobody IGHJ4_2 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_2-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_3-Cys-His

Plasmid

#233328

Purpose

To express nanobody IGHJ4_3 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_3-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ4_4-Cys-His

Plasmid

#233329

Purpose

To express nanobody IGHJ4_4 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_4-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_IGHJ5-Cys-His

Plasmid

#233330

Purpose

To express nanobody IGHJ5 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ5-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-Linker-C6-His

Plasmid

#233331

Purpose

To express nanobody C6 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-Linker-C6-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-L-VHH16-His

Plasmid

#233332

Purpose

To express nanobody VHH16 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-VHH16-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-L-IGHJ4_1-His

Plasmid

#233334

Purpose

To express nanobody IGHJ4_1 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-IGHJ4_1-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pDG04439

Plasmid

#216365

Purpose

xhNup133-Nb2t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup133-Nb2t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

Plasmid

#199356

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

Plasmid

#199363

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS100 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-TEV-MCS

Plasmid

#199353

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-TEV-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA-TEV

Expression

Mammalian

Mutation

WT

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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Showing: 601 - 620 of 990 results

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