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Showing: 1 - 20 of 28 results
  1. Plasmids 101: Secondary Nanobody Toolbox

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    Blog Post
    ...Comparison Advantages of Nanobodies How to Use the Nanobody Toolbox Find Nanobody Plasmids Structural ...anti-mouse and -rabbit IgG secondary nanobodies toolbox. Nanobodies are like tiny antibodies which work...Advantages of Nanobodies Although antibodies have been the titans of immunodetection, nanobodies may be better... with a tagged nanobody. After this pre-incubation step, the primary antibody-nanobody mix can be added...one-step staining with nanobodies vs two-step staining with antibodies. Nanobodies also simplify multicolor...target (compare nanobodies staining in figure 4A vs 4B). How to use the secondary nanobody toolbox Are you...anti-mouse and -rabbit nanobodies. If you’re interested, you can learn more about how nanobodies are identified...
  2. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    Blog Post
    ...ability of its nanobody partner, so it’s important to insert AsLOV2 at a spot in the nanobody that preserves...GFP nanobodies affected binding of their target proteins. The surface-exposed loops of nanobodies seemed...about using nanobodies to control protein activity Read about RANbodies, reporter nanobody fusions Learn...the secondary nanobody toolbox for immunodetection Resources on Addgene.org Find nanobody expression plasmids...proteins, and then elute YFP.  Option #2: Opto-Nanobodies (OptoNBs) purification You skip adding a tag ...Toettcher Lab, OptoNBs are photoswitchable engineered nanobodies whose binding of their target protein is altered...have two key components: an AsLOV2 domain and a nanobody.  AsLOV2 is a light-oxygen-voltage sensing domain...
  3. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    Blog Post
    ...llamas required. The advantages of synthetic nanobodies Nanobodies are small (12-15 kDa) single-domain proteins... antibody, HCab, and nanobody. For more information read our secondary nanobodies blog post.  A rapid...Purification RANbodies: Reporter Nanobody Fusions Plasmids 101: Secondary Nanobody Toolbox Resources on Addgene.org...Nanobodies from camelids have gotten a lot of press for being a promising approach for treatment or prophylaxis...prophylaxis of COVID-19. Researchers identified nanobodies that bind to coronavirus spike proteins (Wrapp...using plasmids to generate sybodies, synthetic nanobodies against the spike protein (Walter et al., 2020... that bind to specific antigens. In contrast, nanobodies are a single heavy domain fragment derived from...
  4. RANbodies: Reporter Nanobody Fusions

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    Blog Post
    ...fusion between a reporter and a nanobody that’s encoded on a plasmid. A nanobody is a ~150 amino acid, single...protein? No worries! As more nanobodies are developed, there will be more nanobodies to turn into RANbodies....More nanobody expression plasmids can be found here Watch this video to learn more about nanobodies Additional...difficult for individual labs to complete. The nanobody based RANbody platform from the Sanes Lab overcomes...RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following antigens...and the actin-binding protein gelsolin, but any nanobody sequence could be used to make a RANbody. RANbodies... MYC tags built into a GFP scaffold. Some GFP nanobodies  stain poorly when tagged with a Spaghetti Monster...
  5. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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    Blog Post
    ...Marcy Patrick  Listen to the nanobody toolkit podcast segment Nanobodies are single domain antibody fragments...Zhao C, et al. RNA. 2018 PubMed PMID:29109157. Nanobody toolkit to monitor retrograde transport at the... as regular antibodies. These properties make nanobodies easier to transform and express in bacteria, ... Spiess lab developed a set of functionalized nanobodies to study retrograde transport from the cell surface... a set of plasmids each containing an anti-GFP nanobody fused with a different functional element (tyrosine...different detection methods. Since the anti-GFP nanobody is able to bind fluorescent proteins derived from... from A. victoria the functionalized nanobodies described here can be used to target any protein fused...
  6. Hot Plasmids - August 2020

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    Blog Post
    ...160374 Nanobodies- there's a simple method for that! By Michelle Cronin The benefits of nanobodies over ...antibodies. Nanobodies are easily expressed in the cytosol of E. coli, however the nanobody proteins tend...evolution Fluorescent tools for mammalian cells Nanobody purification CRISPRi and CRISPRa system for hPSCs... conventional antibodies can’t be overlooked. Nanobodies are smaller in size, more durable, and usually...alleviates the inclusion body problem and makes nanobody production in E.coli as simple as following a ...signal peptide of MBP, followed by the desired nanobody (NB) sequence, an AviTAG, and 6xHis tag. The MBP...supernatant. Four plasmids containing different nanobodies (NB) were generated in pMAK:  pMAK461(NBαRabitb-IgG-Fc...
  7. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    Blog Post
    ... GFP-binding nanobodies, the project really took off. Unlike most antibodies, nanobodies, which occur ...inside cells. Nanobodies binding GFP were designed in 2009. Subsequent work showed that nanobodies could be...and money needed to develop new lines. Using nanobodies to create GFP scaffolds for transcriptional activation...  References 1.Tang, Jonathan C.Y., et al. “A nanobody-based system using fluorescent proteins as scaffolds...Modulation of protein properties in living cells using nanobodies.” Nat. Struct. Mol. Biol. 17 (2010): 133–138....Fluorescent fusion protein knockout mediated by anti-GFP nanobody.” Nat. Struct. Mol. Biol. 19 (2012), 117–121. ...
  8. Trimmer Lab NeuroMab Collection

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    Collection
    ...victoria Mouse Nanobody Plasmid Collection The following plasmids can be used to produce nanobodies from transfected... anti-Homer1 nanobody HS63 Homer1 Mouse Llama 134716 HS69 pComb3xss anti-Homer1 nanobody HS69 Homer1 Mouse... anti-Homer1 nanobody HS89 Homer1 Mouse Llama 134718 HC25 pComb3xss anti-Homer1 nanobody HC25 Homer1 Mouse... anti-Homer1 nanobody HC89 Homer1 Mouse Llama 134720 GC1 pComb3xss anti-Gephyrin nanobody GC1 Gephyrin...anti-Gephyrin nanobody GC83 Gephyrin Mouse Llama 135219 HS22 pEYFPN1 anti-Homer1 nanobody HS22 Homer1 Mouse...pEYFPN1 anti-Homer1 nanobody HC20 Homer1 Mouse Llama 135221 HS38 pEYFPN1 anti-Homer1 nanobody HS38 Homer1 Mouse... anti-Homer1 nanobody HC87 Homer1 Mouse Llama 135224 GC52 pEGFPN1 anti-Gephyrin nanobody GC52 Gephyrin...
  9. Antibodies 101: Introduction to Antibodies

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    Blog Post
    ...Variables (scFvs). Nanobodies Figure 3: Comparison of the Hcab and nanobody. Nanobodies are the variable...synthetic nanobodies, or synbodies, can be produced from plasmids, saving time and money. Nanobodies and synbodies...to reach. While a full antibody is ~150 kDa, a nanobody is 12-15 kDa.   Research applications for antibodies...Collection includes plasmids that express antibodies, nanobodies, and ScFvs, as well as tools for developing and...Developmental Studies Hybridoma Bank Learn more about nanobodies Resources on Addgene.org: Browse Addgene’s ...
  10. Antibody Plasmid Collection

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    Collection
    ..., including monoclonal antibodies, scFvs, and nanobodies....scFvs and single-domain antibodies (also known as nanobodies). For example, scientists often use plasmids ...Sybody Generation Toolbox for making synthetic nanobodies (sybodies) against membrane protein targets. ...Elife. 2018 May 24;7. and Generation of synthetic nanobodies against delicate proteins. Nat Protoc. 2020 Apr...plasmids that feature expression of antibodies, nanobodies or ScFvs. Use the search bar or sort buttons ... antigens such as GFP or mCherry Monoclonals, Nanobodies, Sybodies, or ScFVs Secondary antibodies (search...
  11. Hot Plasmids - May 2022

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    Blog Post
    ... The binding of the GFP nanobody to its target stabilizes the TurboID-nanobody protein and allows TurboID...TurboID fused to a conditionally stabilized GFP-nanobody rather than a specific protein of interest. This...GFP-tagged protein (albeit slightly offset due to the nanobody-GFP interaction). The modularity of the system...
  12. Antibodies 101: Affinity Reagents

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    Blog Post
    ...antibodies (sdAbs), sold under the commercial name nanobodies, are derived from a camelid antibody lacking ...10.1016/j.ymeth.2018.09.005 Muyldermans S. (2013). Nanobodies: natural single-domain antibodies. Annu Rev Biochem...
  13. Hot Plasmids and Viral Preps - March 2021

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    Blog Post
    ...surface and “receiver” cells express an anti-GFP nanobody on the cell surface. When the cells interact, ...fluorophore/antibody pairs. Because the anti-GFP nanobody cross-reacts with BFP, sender cells expressing...
  14. Antibodies

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    Collection
    ...affinity reagent plasmids - including antibodies, nanobodies, scFvs, and more! Find Plasmids...
  15. COVID-19 Resources

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    Collection
    ...available from Andrea Pauli's lab. Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction... Nat Struct Mol Biol. 2020 Jul 13. SARS-CoV-2 Nanobodies now available from Ray Owens' lab. Development...
Showing: 1 - 20 of 28 results