Addgene

We narrowed to 1,257 results for: nanobody

Showing: 1 - 20 of 1257 results

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pCDNA3.0_mitoNanobody

Plasmid

#136622

Purpose

Plasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-EnhancerNb

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoNanobody

Plasmid

#136622

Purpose

Plasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-EnhancerNb

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiex-1 GFPNanobody_SNAP

Plasmid

#82711

Purpose

Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

GFP Nanobody

Use

Tags

6xHis Purification tag, FLAG purification tag, an…

Expression

Bacterial and Insect

Mutation

Promoter

T7 Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiex-1 GFPNanobody_SNAP

Plasmid

#82711

Purpose

Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

GFP Nanobody

Use

Tags

6xHis Purification tag, FLAG purification tag, an…

Expression

Bacterial and Insect

Mutation

Promoter

T7 Promoter

Availability

Academic Institutions and Nonprofits only

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SPYtag-GFPnanobody

Plasmid

#214837

Purpose

This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al bioRxiv. 2024.

Insert

SPYtag-GFPnanobody

Use

Tags

His-TEV-SUMO-

Expression

Bacterial

Mutation

Promoter

T5

Availability

Academic Institutions and Nonprofits only

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pOPINE GFP nanobody

Plasmid

#49172

Purpose

Bacterial expression of GFP nano body

Depositor

Brett Collins

Article

Kubala et al Protein Sci. 2010 Dec;19(12):2389-401. d

Insert

GFP nanobody

Use

Tags

HHHHHH

Expression

Bacterial and Mammalian

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1-GFP-Nanobody

Plasmid

#61838

Purpose

Expresses GST-tagged anti-GFP Nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

GFP-Nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINE GFP nanobody:Halo:His6

Plasmid

#111090

Purpose

Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

Depositor

Lennart Wirthmueller

Article

Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

Insert

GFP Nanobody

Use

Tags

Halo and His6

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.0_nucNanobody IRES EGFP

Plasmid

#136619

Purpose

Plasmid encoding nuclear localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-EnhancerNb-NLS (IRES EGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.0_mitoNanobody IRES EGFP

Plasmid

#136621

Purpose

Plasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-EnhancerNb (IRES EGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.0_LynNanobody IRES EGFP

Plasmid

#136624

Purpose

Plasmid encoding outer inner membrane localization of the Enhancer nanobody GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-EnhancerNb (IRES EGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1-mCherry-Nanobody

Plasmid

#70696

Purpose

Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

mCherry-Nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHEN-CoV2-nanobody-G6

Plasmid

#194589

Purpose

Bacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

G6 (S )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHEN-CoV2-nanobody-E2

Plasmid

#194590

Purpose

Bacterial expression of E2 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

E2 (S )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHEN-CoV2-nanobody-C7

Plasmid

#194591

Purpose

Bacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

C7 (S )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHEN-CoV2-nanobody-F1

Plasmid

#194592

Purpose

Bacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

F1 (S )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINE GFP nanobody

Plasmid

#49172

Purpose

Bacterial expression of GFP nano body

Depositor

Brett Collins

Article

Kubala et al Protein Sci. 2010 Dec;19(12):2389-401. d

Insert

GFP nanobody

Use

Tags

HHHHHH

Expression

Bacterial and Mammalian

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1-mCherry-Nanobody

Plasmid

#70696

Purpose

Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

mCherry-Nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1-GFP-Nanobody

Plasmid

#61838

Purpose

Expresses GST-tagged anti-GFP Nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

GFP-Nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.0_nucNanobody IRES EGFP

Plasmid

#136619

Purpose

Plasmid encoding nuclear localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-EnhancerNb-NLS (IRES EGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge