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Addgene

We narrowed to 691 results for: pseudomonas

Showing: 601 - 620 of 691 results
  1. pEditA

    Plasmid
    #207531
    Purpose
    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Insert
    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pBEAST_J23101-bphC

    Plasmid
    #128137
    Purpose
    The cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032
    Depositor
    Insert
    bphC
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101
    Available since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3

    Plasmid
    #201534
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Insert
    ilvA-relE_RBS3_CB3
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    rhaS W190G
    Promoter
    PrhaBAD (rhamnose)
    Available since
    Sept. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2

    Plasmid
    #201533
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Insert
    ilvA-relE_RBS3_CB2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    rhaR L128+1bp (frameshift), ilvA G455C
    Promoter
    PrhaBAD (rhamnose)
    Available since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4

    Plasmid
    #201535
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Insert
    ilvA-relE_RBS3_CB4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    rhaS ΔM1-H5, PrhaSR Δ179bp
    Promoter
    PrhaBAD (rhamnose)
    Available since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pBEAST_J23101-bphD

    Plasmid
    #128138
    Purpose
    The cell-free adapted backbone, pBEAST, expressing bphD (the enzyme converting 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate to benzoate) under control of the constitutive promoter J23101, and RBS B0032
    Depositor
    Insert
    bphD
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101
    Available since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. pGTPA

    Plasmid
    #171525
    Purpose
    Plasmid contains the Cas12k gRNA and transposon donor cassette
    Depositor
    Insert
    Cas12k gRNA, transposon donor cassette
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Constitutive promoter J23119
    Available since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. pBBR15.2-2H12.D11opt-scarRef

    Plasmid
    #221150
    Purpose
    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)
    Depositor
    Inserts
    cdGreen2.1
    mScarlet-I
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen2.1)
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pGTPA-eGFP

    Plasmid
    #171526
    Purpose
    Plasmid contains the Cas12k gRNA and transposon donor cassette assembled with eGFP
    Depositor
    Insert
    Cas12k gRNA, transposon donor cassette, eGFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Constitutive promoter J23119
    Available since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. pGTPA-Gm

    Plasmid
    #171527
    Purpose
    Plasmid contains the Cas12k gRNA and transposon donor cassette assembled with gentamycin-resistant gene
    Depositor
    Insert
    Cas12k gRNA, transposon donor cassette, gentamycin-resistant gene
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Constitutive promoter J23119
    Available since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. S17-1λpir gyrAR462C

    Bacterial Strain
    #237425
    Purpose
    This engineered E. coli S17-1 λpir strain, featuring a mutation in the gyrA gene (462Arg→Cys), was designed to confer resistance to the CcdB toxin, allowing it to survive with a ccdB-carrying plasmid.
    Depositor
    Bacterial Resistance
    None
    Available since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
  12. pCOLA-A-pC-tac

    Plasmid
    #213494
    Purpose
    Encodes Node A of the three-node Turing circuit
    Depositor
    Inserts
    rpaI (rpaI )
    tetR
    4cl
    tal
    Use
    Synthetic Biology
    Tags
    ASV tag and LVA tag
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 601 - 620 of 691 results