Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
We will increase some of our prices at 12:00 AM ET on April 1, 2023. Be sure to complete your order before this time to take advantage of current prices. See the new prices and get more information or speak with our friendly support team.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Showing: 1 - 20 of 488 results

  3. pPaGE Pyl TAG FliC T248TAG

    Plasmid
    #160089
    Purpose
    Encodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.
    Depositor
    Lital Alfonta
    Article
    Ozer et al Sci Adv. 2021 Jun 11;7(24). pii: 7/24/ea
    Inserts
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Flagellin
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Changed Threonine 248 to TAG stop-codon.
    Promoter
    Pseudomonas aeruginosa Leu-tRNA native promoter a…
    Availability
    Academic Institutions and Nonprofits only
    160089-plasmid-map-sequence-id-314779

    Enlarge

Showing: 1 - 20 of 488 results