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pFTK010

Plasmid

#171282

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PxlnA ANIA_03613 promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-BaeI-Pgk-Cre

Plasmid

#173663

Purpose

Vector with BaeI site to allow cloning of custom sgRNA into vector containing Cre-recombinase

Depositor

Charles Swanton

Publication

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

BaeI site

Use

CRISPR, Cre/Lox, and Lentiviral

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pYFAC-CH2

Plasmid

#168978

Purpose

AMA1 based expression in filamentous fungi pYFAC-pyrG carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

Depositor

Yit Heng Chooi

Publication

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Heterologous expression in asp…

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PB CAG-sfGFP-iCre-ERT2

Plasmid

#137856

Purpose

CAG-sfGFP-iCre-ERT2 piggyBac transposon

Depositor

Mario Capecchi

Insert

sfGFP-iCre-ERT2

Use

Cre/Lox; Piggybac

Expression

Mammalian

Available since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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eGFP L138 gRNA

Plasmid

#119133

Purpose

gRNA that guides Cas9 and APOBEC complexes to L138 in eGFP.

Depositor

Reuben Harris

Publication

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

eGFP L138 gRNA

Use

CRISPR

Mutation

None

Promoter

U6

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAG416Gal-GFP

Plasmid

#38309

Depositor

Susan Lindquist

Publication

McLellan et al ACS Chem Biol. 2012 Jun 22.

Insert

EGFP (eGFP )

Expression

Yeast

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
MSCV-PM mir shLEF1 b

Plasmid

#27018

Depositor

Joan Massague

Publication

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir LEF1 1b

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pFTK048

Plasmid

#171320

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

DsRed.T1-SKL fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK037

Plasmid

#171309

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

bleoR phleomycin, selection marker

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK020

Plasmid

#171292

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PalcA syn_NoCrea (ANIA_08979) promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK017

Plasmid

#171289

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PTET-5x-repeat promoter+CPgpdA (fused)

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK038

Plasmid

#171310

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

hph, hygR, hygromycin selection marker

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK036

Plasmid

#171308

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

amdS ANIA_08777 acetamidase, selection marker

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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p-dCas9-VP64-Hygro

Plasmid

#104405

Purpose

transient expression of dCas9-VP64 fusion protein

Depositor

Stephan Beck

Insert

VP64

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDIV297

Plasmid

#204820

Purpose

AMA1 plasmid with amdS selection marker

Depositor

Uffe Mortensen

Publication

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Insert

amdS

Use

Fungal expression

Expression

Bacterial

Mutation

two silent mutations (nt177T>C, nt879C>T)

Promoter

Aspergillus nidulans trpC promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFTK019

Plasmid

#171291

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PTET-10x-repeat promoter+CPgpdA (fused)

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK084

Plasmid

#171356

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

TtRNA[Met] A.niger Pol-III terminator

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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CHIKV-nsP3-c001

Plasmid

#173086

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

N/A

Tags

mCerulean

Expression

Bacterial

Mutation

A1334-T1493, codon optimized

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pYFAC-riboB

Plasmid

#168975

Purpose

Filamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; riboB

Depositor

Yit Heng Chooi

Publication

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Expression in aspergillus nidu…

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR.sgKras.9

Plasmid

#91894

Purpose

sgRNAs targeting mouse Kras. 3rd generation lentiviral backbone.

Depositor

Wen Xue

Publication

Mou et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Insert

Kras sgRNA

Use

Lentiviral

Promoter

U6

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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