We narrowed to 25,814 results for: Nov
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Plasmid#149659Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30(-10AC1), PsynAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10AC2)_Psyn-sgRNArodA
Plasmid#149660Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30(-10AC2), PsynAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNAmreB
Plasmid#149595Purposeall-in-one CRISPRi vector for targeting B. burgdorferi mreBDepositorInsertdCas9, lacI, sgRNAmreB
ExpressionBacterialPromoterPflaB, PpQE30(-10TC), PsynAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNAftsI
Plasmid#149591Purposeall-in-all CRISPRi vector for targeting B. burgdorferi ftsIDepositorInsertdCas9, lacI, sgRNAftsI
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30(-10TC), PsynAvailable SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pBbdCas9S(-10TC)_Psyn-sgRNArodA
Plasmid#149657Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRExpressionBacterialPromoterPflaB, PpQE30(-10TC), PsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNAflaB
Plasmid#149589Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
ExpressionBacterialPromoterPflaB, PpQE30(-10TC), PsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m2
Plasmid#84721PurposepVR2 but with a G to U mutation in the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
ExpressionBacterialMutationG to U mutation at position 16 of annotated "…Available SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m3
Plasmid#84722PurposepVR2 but with a C to G mutation in the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
ExpressionBacterialMutationC to G mutation at position 22 of annotated "…Available SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR
Plasmid#84718PurposeE. coli RecA promoter followed by preQ1 riboswitch from the 5' UTR of the queC gene from B. subtilisDepositorInsertsE. coli RecA promoter
5' UTR of queC gene
ExpressionBacterialAvailable SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m1
Plasmid#84720PurposepVR2 but with a C to U mutation in the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
ExpressionBacterialMutationC to U mutation at position 13 of annotated "…Available SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG SMAD4
Plasmid#83094PurposeGateway entry vector for inducible expression of human SMAD4 with N-terminal 3xFLAG tagDepositorInsertSMAD4 (SMAD4 Human)
UseEntry vector for gateway cloningTags3xFLAGPromoterTRE tight promoterAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTGC-U6.3
Plasmid#112810PurposePCR template vector for amplifying gRNAcore-pU6.2 promoter fragmentDepositorInsertU6:3-gRNAcore
ExpressionBacterialAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-65/68
Plasmid#113921PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68DepositorInsertMBP-NanoLucCP-65/68
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-65/66
Plasmid#113920PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66DepositorInsertMBP-NanoLucCP-65/66
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-PdhR
Plasmid#89655PurposePlasmid for titrating synthesis of PdhR with doxycyclineDepositorInsertPyruvate dehydrogenase complex repressor
UseSynthetic BiologyAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsB
Plasmid#107559PurposePlasmid for titrating synthesis of FtsB with doxycyclineDepositorInsertftsB
UseSynthetic BiologyPromoterpLtetOAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ121
Plasmid#99325PurposeExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequenceDepositorInsertTALE-VP64-T2A-TALE-VP64
UseTALENExpressionPlantAvailable SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ127B
Plasmid#99883PurposeExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence under 2x35S promoterDepositorInsertTALE-VP64-T2A-TALE-VP64
UseTALENExpressionPlantAvailable SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10
Plasmid#104618PurposeExpresses FLAG tagged Cerulean upon binding of TALE4 to VAS4DepositorInserthsp70-Syn21-FLAG-mCerulean-P10
TagsFLAGExpressionInsectPromoterhsp70Available SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only