We narrowed to 3,364 results for: guide rna expression plasmid

#160734

Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

Samuel H. Sternberg

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Dec. 1, 2020

Academic Institutions and Nonprofits only

#160735

Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

Samuel H. Sternberg

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Nov. 16, 2020

Academic Institutions and Nonprofits only

#131774

Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.

Frank Albert

Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

Oct. 7, 2019

Academic Institutions and Nonprofits only

#89898

Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

Feng Zhang

Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

May 12, 2017

Academic Institutions and Nonprofits only

#89906

Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

Feng Zhang

Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

May 12, 2017

Academic Institutions and Nonprofits only

#229847

Expresses wild-type Cas9 and scrambled gRNA.

Natsuko Chiba

Jan. 9, 2025

Academic Institutions and Nonprofits only

#86620

Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

Yannick Doyon

Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

March 16, 2017

Academic Institutions and Nonprofits only

#86619

Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

Yannick Doyon

Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

March 16, 2017

Academic Institutions and Nonprofits only

#180605

Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX

Jennifer Doudna

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

April 25, 2022

Academic Institutions and Nonprofits only

#180606

Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX

Jennifer Doudna

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

April 25, 2022

Academic Institutions and Nonprofits only