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We narrowed to 24,340 results for: Fus

Showing: 6201 - 6220 of 24340 results
  1. pU6-(BsaI)_CBh-UN-Cas9

    Plasmid
    #135011
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9
    Depositor
    Insert
    humanized S. pyogenes Cas9
    Tags
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pDual

    Plasmid
    #207883
    Purpose
    Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.
    Depositor
    Inserts
    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
    Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Leucine 212 changed to proline, leucine 434 in th…
    Available Since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pCS2+mNeonGreen-C Cloning Vector

    Plasmid
    #128144
    Purpose
    To express a protein of interest fused to the C-terminus of mNeonGreen
    Depositor
    Type
    Empty backbone
    Tags
    mNeonGreen fusion
    Expression
    Mammalian
    Available Since
    July 2, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pCS2+mScarlet-C Cloning Vector

    Plasmid
    #128147
    Purpose
    To express a protein of interest fused to the C-terminus of mScarlet
    Depositor
    Type
    Empty backbone
    Tags
    mScarlet fusion
    Expression
    Mammalian
    Available Since
    July 2, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pZP06B

    Plasmid
    #232321
    Purpose
    Make sacB a counterselection marker to replace galK
    Depositor
    Insert
    PrpsJ-sacB
    Expression
    Bacterial
    Available Since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pBCG10

    Plasmid
    #232323
    Purpose
    Make mazF a counterselection marker to replace sacB in pZP06C
    Depositor
    Insert
    mazF-tetR
    Expression
    Bacterial
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA3.1-hSyncytin-1(deltaFP)

    Plasmid
    #182443
    Purpose
    hSyncytin-1, fusion peptide deletion(320-340)
    Depositor
    Insert
    ERVW-1 (ERVW-1 Human)
    Expression
    Mammalian
    Mutation
    no fusion peptide (320-340)
    Promoter
    CMV-F
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. pCSD_SpCas9MT3-FKBP_SL

    Plasmid
    #107302
    Purpose
    Expresses R1335K mutant SpCas9 fused to FKBP in mammalian cells
    Depositor
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    FKBP
    Expression
    Mammalian
    Mutation
    R1335K and fused to FKBP
    Promoter
    CMV IE94
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL

    Plasmid
    #107305
    Purpose
    Expresses R1335K mutant SpCas9 fused to FKBP with extra NLS in mammalian cells
    Depositor
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    2x NLS and FKBP
    Expression
    Mammalian
    Mutation
    R1335K and fused to FKBP
    Promoter
    CMV IE94
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. pMin1

    Plasmid
    #102959
    Purpose
    Mini-transposon system suitable for sequencing, shuttle mutagenesis and gene fusions
    Depositor
    Type
    Empty backbone
    Use
    Mini-transposon system suitable for sequencing, s…
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 6201 - 6220 of 24340 results