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EC.SurA.pTYB1.a21-281&390-428.wt

Plasmid

#12607

Depositor

David McKay

Article

Bitto et al J Biol Chem. 2003 Dec 5. 278(49):49316-2

Insert

Escherichia coli surA delta PPIase domain 2

Tags

intein

Expression

Bacterial

Mutation

residues 282-389 deleted

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pTAL2

Plasmid

#31033

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

TALE fusion

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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TAL2368

Plasmid

#36779

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

NCOR1-TALEN_Left (Ncor1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pRS405 VAC8-RFP

Plasmid

#166841

Purpose

Expresses Vac8-RFP in yeasts

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

RFP

Expression

Yeast

Promoter

VAC8 promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTAL3

Plasmid

#31034

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

TALEN-FokI nuclease backbone

Tags

AcV5 and FokI nuclease

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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His6_BcLOV4_Q355N_mCherry_BamUK

Plasmid

#119762

Purpose

Encodes mCherry-tagged BcLOV4 permanently-"lit" mutant with Q355N mutation for expression in bacterial cell

Depositor

Brian Chow

Article

Glantz et al Methods Enzymol. 2019;622:249-270. doi:

Insert

BcLOV4-Q355N_mCherry

Tags

mCherry

Expression

Bacterial

Mutation

Mutated glutamine at position 355 to asparagine

Promoter

T7

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pNG2

Plasmid

#30986

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NG2

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pPyCAG-Myc-ERas-IP

Plasmid

#15690

Depositor

Shinya Yamanaka

Article

Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

Insert

ERas (Eras Mouse)

Tags

Myc (6x)

Expression

Mammalian

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pHJ346

Plasmid

#20678

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Moesin (MSN Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Lys 253, 254, 262, 263 to Asn

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
GFP-Rap1b S179AS180A

Plasmid

#118327

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

Insert

Rap1b S179AS180A (RAP1B Bovine)

Tags

GFP

Expression

Mammalian

Mutation

S179AS180A

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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BSP602

Plasmid

#122249

Purpose

expression vector for mScarlet under EFT3 Promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mScarlet

Use

Synthetic Biology

Expression

Worm

Promoter

EFT3

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTK52_GFP-NuMA-C-3A

Plasmid

#46350

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

NuMA C-terminal fragment (NUMA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

C-terminal fragment from 1700-2115, and changed T…

Promoter

pBABE

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pGZ12.0501

Plasmid

#141164

Purpose

TcTT2 in Ti plasmid binary vector pGZ12.0501 (KF871320.1)

Depositor

Mark Guiltinan

Article

Florez et al BMC Plant Biol. 2015 May 16;15:121. doi:

Insert

TcTTC

Expression

Plant

Promoter

E12-omega

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDD375

Plasmid

#91825

Purpose

Codon-optimized mKate2 donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

mKate2-C1

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDTnLAPP2A frame 0

Plasmid

#28226

Depositor

Harald von Melchner

Article

Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

Insert

Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein

Use

Retroviral

Tags

EGFP and nLAP-tag

Available Since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pTub-alpha1-iLifeact-NeonGreen

Plasmid

#175258

Purpose

F-actin structures labelling

Depositor

Frank Bradke, Sebastián Dupraz

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

iLifeact

Tags

mNeonGreen

Expression

Mammalian

Mutation

None (wt)

Promoter

pTub-alpha1

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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M-R742X (BF7-2)

Plasmid

#21372

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type II (PKD2 Human)

Tags

6xMyc

Expression

Mammalian

Mutation

changed R742 to stop codon

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pMaCTag-28

Plasmid

#120007

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xHA

Use

Pcr template

Tags

3xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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YCe1905 HC_Kan_DmrA_p21

Plasmid

#100685

Purpose

part designed to occupy position 21 of EMMA. Functional category: Dimerization domain

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

DmrA

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2052 HC_Kan_DmrC_p9

Plasmid

#100693

Purpose

part designed to occupy position 9 of EMMA. Functional category: Dimerization domain

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

DmrC

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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