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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 6261 - 6280 of 6562 results

  1. MCP-Rep-X-RT∆R

    Plasmid
    #239384
    Purpose
    For circular RNA-mediated inverse prime editor using MCP-Rep-X-M MLV-RT∆RNase H in HEK294T cells
    Depositor
    Caixia Gao
    Publication
    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MCP, Rep-X, M-MLV-RT(∆RNase H)
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ∆RNase H in M-MLV RT
    Promoter
    CMV
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239384-plasmid-map-sequence-id-481789

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  3. pYN2_1

    Plasmid
    #184757
    Purpose
    Plasmid for genome editing by CRISPR/Cas9
    Depositor
    Yvonne Nygård
    Publication
    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10
    Inserts
    Cas9
    sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
    Use
    Synthetic Biology
    Tags
    2xNLS
    Expression
    Bacterial and Yeast
    Promoter
    PGK1 and tRNA-Phe-HDV Ribozyme
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    184757-plasmid-map-sequence-id-421882

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  4. Bxb1-GA_AIO-TetOn-Ngn2_TetOn_ISL1-LHX3_Divergent

    Plasmid
    #250433
    Purpose
    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of Ngn2, ISL1 and LHX3
    Depositor
    Richard Davis, Kate Galloway
    Publication
    Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103
    Inserts
    Ngn2 (NEUROG2 Human)
    ISL1-T2A-LHX3 (ISL1, LHX3 Human)
    TetOn3G rtTA
    Tags
    3xHA and T2A-mTagBFP2-3xNLS
    Expression
    Mammalian
    Promoter
    CAG and TRE3G
    Available since
    April 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    250433-plasmid-map-sequence-id-497778

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  5. PB-3xFLAG-2xStrepTag

    Plasmid
    #253239
    Purpose
    Empty plasmid or Plasmid control for the plasmids: "CAGp_LYST-3xFLAG-2xStrepTag_PiggyBacTransposon" and "CAGp_3xFLAG-2xStrep-Tag_LYST_PiggyBacTransposon".
    Depositor
    May Malicdan
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    3xFLAG-2xStrepTag
    Expression
    Mammalian
    Available since
    May 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    253239-plasmid-map-sequence-id-503853

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  6. PB-3xFLAG

    Plasmid
    #253240
    Purpose
    Empty plasmid or Plasmid control.
    Depositor
    May Malicdan
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    3xFLAG
    Expression
    Mammalian
    Available since
    May 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    253240-plasmid-map-sequence-id-503912

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  10. pCMV-T7-SpCas9(MRKCRS)-P2A-EGFP (RAS2821)

    Plasmid
    #223102
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized nuclease SpCas9(MRKCRS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(MRKCRS)=D1135M, S1136R, G1218K, E1219C, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223102-plasmid-map-sequence-id-447669

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  11. pCMV-T7-SpCas9(LWRYEK)-P2A-EGFP (RAS2986)

    Plasmid
    #223094
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized nuclease SpCas9(LWRYEK)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(LWRYEK)=D1135L, S1136W, G1218R, E1219Y, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223094-plasmid-map-sequence-id-447661

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  12. pCMV-T7-SpCas9(MKRCMV)-P2A-EGFP (AHK162)

    Plasmid
    #223075
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized nuclease SpCas9(MKRCMV)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(MKRCMV)=D1135M, S1136K, G1218R, E1219C, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223075-plasmid-map-sequence-id-447667

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  13. pCMV-T7-SpCas9(MRRWMR)-P2A-EGFP (RAS2816)

    Plasmid
    #223068
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized nuclease SpCas9(MRRWMR)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223068-plasmid-map-sequence-id-447673

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Showing: 6261 - 6280 of 6562 results