We narrowed to 32,755 results for: eng

1. pML460_MAP1LC3B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227901

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   MAP1LC3B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pML349_PEX3-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227902

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   PEX3 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227904

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   SEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227905

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   COPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60225

   Purpose

   Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA targeting LacZ. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Renilla luciferase
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

   Tags

   Cre-HA and Rluc-P2A

   Expression

   Mammalian

   Promoter

   EFS and U6

   Available Since

   Oct. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122

   Plasmid

   #223184

   Purpose

   Plasmid containing the Zfr/Firefly luciferase/3xMIR122 cassette. Translation of luciferase is controlled by splicing modulation of the Zfr specifically in cardiomyocytes by risdiplam in vivo.

   Depositor

   Yuxuan Guo

   Article

   Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

   Inserts

   luciferase (LOC116160065 firefly)
   mir122 (Mir122 Mouse)
   

   Use

   AAV

   Tags

   HA and engineered P2A

   Promoter

   TNNT2

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Icam 2 SaCas9 YAP sgRNA1

   Plasmid

   #99734

   Purpose

   Expresses SaCas9 and a gRNA targeting mouse YAP

   Depositor

   Yu Huang

   Article

   Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

   Insert

   SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA

   Plasmid

   #197031

   Purpose

   Vector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized denOsCas12f1-VPR
   

   Expression

   Mammalian

   Mutation

   D52R / T132R / D228A / D406A

   Promoter

   CBh, CMV, hU6

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZE21/UBP1/ClpS_V65I

   Plasmid

   #98567

   Purpose

   Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon

   Depositor

   George Church

   Article

   Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

   Inserts

   Truncated ubiquitin cleavase, codon optimized
   Mutated ATP-dependent Clp protease adapter protein
   

   Expression

   Bacterial

   Mutation

   Contains the V65I mutation that increases discrim…

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6xHis-MBP-BrCas12b-FNLDTA

    Plasmid

    #195340

    Purpose

    Expresses Engineered BrCas12b in Bacteria

    Depositor

    Piyush Jain

    Article

    Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

    Insert

    BrCas12b
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis Tag and MBP, TEV cleavage site, 6x His Tag

    Expression

    Bacterial

    Mutation

    F208W, N524V, L795I, D868V, T874S, A1015E

    Promoter

    T7 Promoter

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Icam 2 SaCas9 YAP sgRNA2

    Plasmid

    #99735

    Purpose

    Expresses SaCas9 and a gRNA targeting mouse YAP

    Depositor

    Yu Huang

    Article

    Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

    Insert

    SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-ecDHFR-enOsCas12f1-NLS-ecDHFR_pA_AAV2 ITR

    Plasmid

    #197029

    Purpose

    AAV vector encoding a human codon-optimized ecDHFR-enOsCas12f1-ecDHFR driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized ecDHFR-enOsCas12f1-ecDHFR
    

    Use

    AAV

    Mutation

    D52R / T132R

    Promoter

    MHCK7, hU6

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSFDuet-FBXL5-SKP1

    Plasmid

    #226620

    Purpose

    Co-expression of FBXL5-SKP1 complex in E.coli

    Depositor

    Ning Zheng

    Article

    Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

    Inserts

    FBXL5 (FBXL5 Human)
    SKP1 (SKP1 Human)
    

    Tags

    GB1

    Expression

    Bacterial

    Mutation

    Amino acids 1-198 was deleted, and 420-596 was re…

    Promoter

    T7

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKan cpRBP-FL P53

    Plasmid

    #174504

    Purpose

    Express Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)

    Depositor

    Stewart Loh

    Article

    Ha et al Bio Protoc. 2021 Oct 20;11(20):e4188. do

    Insert

    P53 gene (TP53 Human)
    

    Tags

    20 amino acid linker containing HRV3C protease si…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)

    Plasmid

    #169189

    Purpose

    Baculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

    Insert

    3xFlag-His6-nsp13 (ORF1ab Synthetic, SARS-CoV-2)
    

    Tags

    3xFlag-His6

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVRC8400-SARS-CoV-2-S2P-B.1.617.2-AVI

    Plasmid

    #176334

    Purpose

    Expresses SARS-CoV-2 S2P protein from the B.1.617.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.617.2 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19R, G142D, del156-157, R158G, L452R, T478K, D61…

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVRC8400-SARS-CoV-2-RBD-BA.2-AVI

    Plasmid

    #184533

    Purpose

    Expresses SARS-CoV-2 RBD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-BA.2 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G339D, S371F, S373P, S375F, T376A, D405N, R408S, …

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVRC8400-SARS-CoV-2-RBD-E484K-AVI

    Plasmid

    #176444

    Purpose

    Expresses SARS-CoV-2 RBD domain containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-E484K (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    E484K

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQhsCDtRNA+

    Plasmid

    #86014

    Purpose

    Multi-gene expression system for purification of human box C/D RNPs with U14-tRNA minigene

    Depositor

    Hong Li

    Article

    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:

    Inserts

    U14-tRNA chimera (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    

    Use

    Co-expression; purification

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    amino acids 1-401, amino acids 1-411, and amino a…

    Promoter

    LPP and Ptac

    Available Since

    Jan. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. CCR5-SZ190b-sfGFP

    Plasmid

    #162447

    Purpose

    Expression in HEK293T cell and compete ligand singaling against full-length receptors, the truncated receptor can perform signaling at high ligand concentration

    Depositor

    Shuguang Zhang

    Article

    Qing et al iScience. 2020 Oct 28;23(12):101670. doi

    Insert

    C-C chemokine receptor type 5 (CCR5 Human)
    

    Expression

    Mammalian

    Mutation

    truncation from aa88 to aa249

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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